Chromatography

Question 1

What are the phases in chromatography and what does each term mean?

Answer 1

Mobile and stationary

Mobile=solvent
Stationary=part of chromatography that doesn’t move e.g paper

Question 2

If you a substance is more soluble what is the attraction in each phase?

Answer 2

Mobile phase=highly attracted
Stationary=lowly attracted

Question 3

How do you calculate RF and what does it stand for?

Answer 3

Retention factor

Distance moved by the solvent

Question 4

Why are RF values important?

Answer 4

Always the same if you do the experiment in the same way with the same substance in the same solvent

This allows us to compare it with other samples

Tells relative solubility of each substance

Max value of the RF would be 1

Question 5

What is used to drop the substance on paper chromatography?

Answer 5

Capillary tube

Question 6

What do you do after the chromatography?

Answer 6

Draw a line for distance of the solvent and let the paper dry out to then find RF values

Question 7

What are the known substances in the practical in terms of purity?

Answer 7

Pure substances