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CHEMISTRY > Chromatography > Flashcards

Chromatography Flashcards

1
Q

What is the mobile phase

A

Solvent

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2
Q

Solvent is

A

Mobile phase

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3
Q

Stationary phase is

A

What sample is separated on

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4
Q

TLC stands for

A

Thin layer chromatography

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5
Q

How are substances separated in TLC

A

•differences in affinities in stationary phase
•differences in solubilities in mobile phase

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6
Q

How are substances separated in column chromatography

A

•differences in affinities in stationary phase
•differences in solubilities in mobile phase

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7
Q

How are substances separated in gas chromatography

A

•differences in affinities in stationary phase

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8
Q

Stationary phase in TLC is

A

Silica plate

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9
Q

Stationary phase in column chromatography is

A

Silica powder

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10
Q

Stationary phase in gas chromatography is

A

Silica powder/resin coated within column

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11
Q

Mobile phase in gas chromatography

A

Inert carrier gas eg N2 or He

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12
Q

How does TLC record

A

Rf value

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13
Q

How does gas chromatography record

A

Retention time and relative abundance

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14
Q

Suitable reagent and condition for hydrolysis

A

Conc HCl
Heat

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15
Q

Why might you need two different solvents

A

Some compounds may not dissolve in one solvent

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16
Q

Name a suitable developing agent

A

Ninhydrin

17
Q

Why do we use developing agents

A

To make amino acids more visible

18
Q

Why is a lid needed for TLC

A

•prevents solvent from evaporating
•creates saturated environment

CHEMISTRY (12 decks)

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