Chromatography Flashcards
Chromatography
separation technique in which a mobile phase carrying a mixture moves in contact with a selectively absorbent stationary phase.
Principle of gas chromatography
a gaseous mobile phase is in contact with a stationary phase and separation of the mixture occurs as a result of selective absorbance of the components of the mixture on the stationary phase.
Principle of high-performance liquid chromatography
a liquid mobile phase is in contact with a stationary phase and separation of the mixture occurs as a result of selective absorbance of the components of the mixture on the stationary phase.
Principle of infra-red spectrometry
organic compounds absorb infra-red radiation of certain frequencies.
Principle of ultraviolet absorption spectrometry
absorbance is directly proportional to the concentration of a substance