Chromatography: Paper Chromatography

Question 1

Chromatography is a technique that can be used to what?

Answer 1

To separate a mixture into its individual components

Question 2

Chromotography relies on what?

Answer 2

Differences in the solubility of the different chemicals within a mixture

Question 3

What are the two phases of chromatography?

Answer 3

1. The mobile phase
2. The stationary phase

Question 4

What do the components in the mixture do as the mobile phase travels over the stationary phase?

Answer 4

Separate

Question 5

What does difference in the solubility of each component in the mobile phase affect?

Answer 5

How far each component can travel

Question 6

Will components with higher solubility travel further or less than the others?

Answer 6

Further

Question 7

Why do components with a higher solubility tavel further than the others?

Answer 7

They spend more time in the mobile phase and are then carried further up the paper

Question 8

What is the stationary phase in paper chromatography?

Answer 8

The chromatography paper

Question 9

What is the mobile phase in paper chromatography?

Answer 9

The solvent (water or ethanol)

Question 10

What is the method of paper chromatography?

Answer 10

1. A spot of the mixture (that you want to separate) is placed on chromatography paper and left to dry
2. The chromatography paper is then suspended in a solvent
3. As the solvent travels up through the chromatography paper, the different components within the mixture begin to move up the paper at different speeds
   - larger molecules move slower than smaller ones
   - this causes the original mixture to separate out into different spots or bands on the chromatography paper
4. This produces what is known as a chromatogram