Chromosomal Karyotype

Question 1

Diagnoses disorders from a missing/additional chromosome

Answer 1

Chromosomal Karyotype

Question 2

Chromosome Labeling

Answer 2

n (p/q) r.b

Question 3

Sample taken from the fluid of the amniotic sac

Answer 3

Amniocentesis

Question 4

Sample taken from the fetal tissue that forms part of the placenta

Answer 4

Chorionic Villus Sampling

Question 5

What phase of mitosis are chromosomes viewed under Light Microscopy

Answer 5

Metaphase

Question 6

Traditional types of staining

Answer 6

G-Baning
Q-Banding
R-Banding

Question 7

Classic method of staining

Answer 7

Homogenous staining by Giemsa

Question 8

Dark stained bands in G-bands

Answer 8

Adenine-Tyrosine

Question 9

Pale stained bands in G-bands

Answer 9

Guanine-Cytosine

Question 10

Dark stained bands in R-bands

Answer 10

Guanine-Cytosine

Question 11

Pale stained bands in R-bands

Answer 11

Adenine-Tyrosine

Question 12

Transcriptionally inactive part of chromosome

Answer 12

Telomere

Question 13

Transcriptionally active part of chromosome

Answer 13

Euchromatin

Question 14

Dark stained in C-banding

Answer 14

Resistant heterochromatin

Question 15

Pale stained in C-banding

Answer 15

Euchromatin parts

Question 16

Stops mitosis in metaphase

Answer 16

Colcemid / Colchine

Question 17

Stimulate mitosis when making a Karyotype

Answer 17

Phytohemagglutinin

Question 18

Technique that hybridizes a DNA nuclei acid probe to a target DNA sequence contained w/in a cell nucleus

Answer 18

Fluorescence In-Situ Hybridization

Question 19

Nucleic acid that can be labeled w/ a marker used for identification & quantitation

Answer 19

Probe

Question 20

Part of DNA/RNA that is complementary to certain sequence on target DNA

Answer 20

Probe

Question 21

Combination of Phase & Plasmid DNA

Answer 21

Cosmid

Question 22

Amplification of certain segment of chromosomal DNA

Answer 22

PCR-product

Question 23

Biotinylated is also labeled as

Answer 23

Avidin-Streptavidin

Question 24

Probe that has specificity for a single human chromosome arm

Answer 24

Telometric Probe

Question 25

Hybridized probe fluoresces w/ bright intensity along the length of the chromosome

Answer 25

Whole Chromosome Painting

Question 26

Probe that makes centromere regions stained brighter

Answer 26

Chromosome Enumerator Probe

Question 27

Formation of a duplex b/w 2 complementary sequences

Answer 27

Nucleic Acid Hybridization

Question 28

b/w 2 polynucleotide chains w/ complementary bases

Answer 28

Intermolecular Hybridization

Question 29

Example of machine for Automated Hybridization

Answer 29

HYBrite

Question 30

FISH results time

Answer 30

< 24hrs

Question 31

Karyotyping results time

Answer 31

7- 10 days

Question 32

PCR was invented in what year

Answer 32

1984

Question 33

Laboratory version of DNA Replication in cells

Answer 33

Polymerase Chain Reaction

Question 34

Occurs outside the body

Answer 34

In Vitro

Question 35

Occurs within the body

Answer 35

In Vivo

Question 36

Catalyzes the elongation of DNA by adding nucleoside triphosphates to the 3' end of the growing strand

Answer 36

DNA Polymerase

Question 37

Enzyme that can start an RNA chain from scratch

Answer 37

Primase

Question 38

Untwists the 2 parallel DNA strands

Answer 38

Helicase

Question 39

Relieves the stress of twisting

Answer 39

Topoisomerase

Question 40

Taq DNA Polymerase is purified from what hot spring bacterium

Answer 40

Thermus Aquaticus

Question 41

Size of DNA fragment produced in PCR is dependent on what?

Answer 41

Primers

Question 42

DNA of interest is amplified by power of # for each PCR cycle whats #?

Answer 42

2

Question 43

HD gene has a pattern called trinucleotide repeats w/ ''CAG'' occurring in repetition <30 times

Answer 43

Non-HD Individuals

Question 44

''CAG'' trinucleotide repeat occurs >36 times

Answer 44

HD Individuals

Question 45

Mutations in the cystic fibrosis transmembrane conductance regulator (CTFR) gene

Answer 45

Cystic Fibrosis

Question 46

codes for a protein that is a chloride ion channel

Answer 46

Cystic Fibrosis Transmembrane Conductance Regulatory gene

Question 47

Application of PCR in Forensic Science

Answer 47

Paternity Suits