CK

Question 1

Total creatine kinase-Kinetic method

Answer 1

Oliver-Rosalki, modified by Szasz

Question 2

Addittion of Sulfhydryl and AMP

Answer 2

Nielson

Question 3

Optimized the condition for measuring CK activity

Answer 3

Szasz and Scandinavian committee

Question 4

Recommended method of IFCC

Answer 4

Reverse reaction and activation of NAC

Question 5

pH of forward reaction

Answer 5

pH 6.8

Question 6

pH of reverse reaction

Answer 6

pH 9.0

Question 7

Activator of CK

Answer 7

Magnesium

Question 8

Enzymes of forward reaction

Answer 8

CK, Pyruvate Kinase, Lactate dehydrogenase

Question 9

Enzymes of reverse reaction

Answer 9

CK, Hexokinase, Glucose-6-Phosphate Dehydrogenase

Question 10

Final product of forward reaction

Answer 10

NADH

Question 11

Final product of reverse reaction

Answer 11

NAD

Question 12

Direct or forward reaction method

Answer 12

Tanzer-Gilbarg method

Question 13

Methods of separation of isoenzyme

Answer 13

Electrophoresis
Immunoassay
Column chromatography

Question 14

Excess magnesium, chloride, calcium, sulfates

Answer 14

Inhibit CK activity

Question 15

Heavy metals that inhibits CK activity

Answer 15

zinc, copper, manganese

Question 16

Excess iodoacetate, and sulfhydryl agents

Answer 16

Inhihits CK activity

Question 17

Excess ADP, citrate, fluoride, L-thyroxine, and uric acid

Answer 17

Inhibits CK activity

Question 18

4hrs
8-12hrs
2-3 days

Answer 18

RT
4 deg celcius
Frozen

Question 19

High ALP

Answer 19

Decrease CK value

Question 20

Inactivated by light

Answer 20

CK activity

Question 21

Lipemic sample

Answer 21

Causes high absorbance readings

Question 22

Intramuscular injection of sedative and analgesics

Answer 22

Induce increase CK activity

Question 23

Men have higher CK than women because of difference in?

Answer 23

Muscle mass

Question 24

New born high value of CK due to

Answer 24

Skeletal muscle trauma during birth

Question 25

Recent exercise

Answer 25

Increase serum CK

Question 26

Sustained exercise

Answer 26

Increase CK-MB

Question 27

Elders have decrease CK due to

Answer 27

Decrease muscle mass

Question 28

Linearity of Total creatine kinase-Kinetic method

Answer 28

1200 IU/L

Question 29

Magnesium

Answer 29

Cofactor for maximum catalytic activity

Question 30

Adenosine-5'-Monophosphate

Answer 30

Inhibits adenylate kinase activity

Question 31

N-acetylcysteine

Answer 31

Prevents oxidation of sulfhydryl group

Question 32

Oxidation of sulfhydryl leads to

Answer 32

No enzyme activity of CK

Question 33

EDTA

Answer 33

Chelates calcium which is an inhibitor of CK

Question 34

55 deg celcius

Answer 34

No detectable CK activity

Question 35

Preffered method of reaction

Answer 35

Reverse reaction

Question 36

This reaction is 2-6× faster

Answer 36

Reverse reaction

Question 37

CK- MB determination Immuno-inhibition

Answer 37

Wurzburg

Question 38

Major source of CK

Answer 38

Muscle- CK-MM Heart- CK- MB Brain- CK-BB

Question 39

Subunits of CK

Answer 39

M and B

Question 40

Isoenzyme are separated via

Answer 40

Electrophoresis Column anion exchange Batch anion exchange chromatography

Question 41

Antibody to CK- M monomer

Answer 41

Wurzburg

Question 42

Quantitatively measure CK- B

Answer 42

Rosalki

Question 43

Uses precipitating antisera against ( homologous rabbit antisera ) pure CK-MM and CK-BB

Answer 43

Immunoprecipitation

Question 44

Used for fast and quantitative measurement of CK-MB

Answer 44

Immuno- inhibition

Question 45

Used in doubtful cases

Answer 45

Immunoprecipitation

Question 46

Isoenzyme that is usually absent is a healthy sera

Answer 46

CK-BB

Question 47

Linearity of CK-MB determination

Answer 47

1500 IU/ L

Question 48

Measurement of isoenzyme

Answer 48

Radio immunoassay Ion exchange chromatography Immuno inhibition Electrophoresis