Clearing Flashcards
(30 cards)
Other term for clearing
De-alcoholization
Process of removing alcohol or dehydrating agent from tissue and replace it with a substance that will dissolve the wax
Clearing
T/F
All clearing Agents give a transparent appearance
False
-chloroform can’t
clearing agents will make tissues transparent due to their?
High index refractivity
Clearing agents used in FSB
Glycerin and gum syrup
T/F
Clearing agents should be miscible with alcohol and paraffin
True
T/F
Most clearing agents are flammable
True
Mnemonics for characteristics of a good clearing agent
MM-PEMD
Factors affecting the rate of penetration of CA
Boiling point
Viscosity
Prolonged exposure to CA will cause
Brittleness (pag clear gani murag glass, DALI RA MABUAK)
Commonly used CA
Xylene
T/F
Xylene is not recommended for brain and CNS Tissues
True
CA recommended for urgent biopsies
Benzene
Carcinogenic CA
Benzene
CA that can be used in tissue blocks even up to 1cm in thickness
Chloroform
CA that don’t become translucent
Chloroform
Recommended for tough tissues
Chloroform
CA that can cause liver damage
Chloroform
Tissue stem to float in this CA
Chloroform
-wrap with gauze to facilitate sinking
Recommended for brain and CNS tissues, cytologic studies, smooth muscle and skin
Cedarwood oil
Cedarwood oil can clear tissues in how many days?
Paraffin- 2-3 days
Celloidin- 5-6 days
This chemical hasten the removal of cedarwood oil
Benzene
Becomes milky upon storage
Cedarwood oil
Tissue initially float in this chemical
Cedarwood
-superimpose absolute alcohol as remedy
