Infiltration & Embedding

Question 1

The process of completely removing the clearing agent and replacing it with a medium that will completely fill all the tissue cavities

Answer 1

Infiltration /impregnation

Question 2

Purpose of infiltration and embedding?

Answer 2

Give firm consistency and allow easier handling and cutting

Question 3

Other term for embedding

Answer 3

Blocking/casting

Question 4

T/F

The medium used in infiltration is almost always the same with the medium used in embedding

Answer 4

True

Question 5

Simplest and most common embedding medium

Answer 5

Paraffin wax

Question 6

Allow serial section cutting

Answer 6

Paraffin wax

Question 7

Melting point of paraffin wax

Answer 7

56-57*C

Question 8

Paraffin can’t infiltrate

Answer 8

BeeBee

Bones
Teeth
brain
eyes

Question 9

This EM is not recommended for fatty tissues

Answer 9

Paraffin wax

Question 10

Overheated paraffin will cause

Answer 10

Brittleness

Too hot = marupok

Question 11

Melting points of common waves

Answer 11

45, 52, 56, 58*C

Question 12

Recommended MP of paraffin waxes according to lab temp

Answer 12

20-24*C = 54-58*C
15-18*C = 50-54 *C

Question 13

T/F

👆🏻melting point = harder wax = easier cutting

Answer 13

True

Question 14

T/F

The harder the wax the more thinner the sections it can produce

Answer 14

True

Question 15

T/F

Harder tissue require harder wax

Answer 15

True

Question 16

3 ways of paragon wax infiltration

Answer 16

Manual (4)
Automatic (2-3)
Vacuum (3)

Question 17

Give the needed temp for infiltration in each method

Answer 17

Manual - 2-5C
Automatic - 3C
Vacuum -2-4*C

Question 18

Other term for automatic tissue processor

Answer 18

Autotechnicon

Question 19

T/F

Autotechnicon has vacuum which makes impregnation faster

Answer 19

False

-constant agitation

Question 20

How many breakers are used in autotechnicon?

Answer 20

12
10- glass
2- thermostatically controlled wax baths

Question 21

This method involves wax impregnation under negative atmospheric pressure (vacuum)

Answer 21

Vacuum embedding

Question 22

Most rapid method

Answer 22

Vacuum

-reduce 25-75% of the normal time required for tissue processing

Question 23

Factors affecting paraffin wax impregnation

Answer 23

1. Nature & size of tissue

2. Type of clearing agent

Question 24

Temp above _______ temp will cause considerable damage to tissue

Answer 24

60*C

Question 25

What to do before using paraffin

Answer 25

Melt at 2*C above MP then filter using Green’s no. 904

Question 26

What to if if paragon wax is contaminated with water?

Answer 26

Heat 100-105*C

Question 27

Mediums that can be used in impregnation

Answer 27

Paraffin Celloidin Gelatin

Question 28

Paraffin should be melted at what temp?

Answer 28

5-10*C above MP

Question 29

The process by which tissue is arranged in a precise position

Answer 29

Orientation

Question 30

3 ways of solidifying the block

Answer 30

Ref @ 5*C Cold water Cold plate