Clinical Chemistry

Question 1

Measures light emitted by a single atom burned in a flame

Answer 1

FLAME EMISSION PHOTOMETRY

Question 2

Measures Light absorbed by atoms in a ground state dissociated bu heat

Answer 2

ATOMIC ABSORPTION SPECTROPHOTOMETRY

Question 3

light source of AAS

Answer 3

HOLLOW-CATHODE LAMP

Question 4

used for excited ions

Answer 4

FLAME EMISSION PHOTOMETRY

Question 5

used for unexcited trace elements (Ca, Mg)

Answer 5

AAS

Question 6

uses an LED lamp which generates light which passes through a filter and a series of slits

Answer 6

REFLECTANCE PHOTOMETRY

Question 7

unknown sample is made to react with known solution

Answer 7

VOLUMETRIC (TITRIMETRIC)

Question 8

Examples of volumetric

Answer 8

SCHALES AND SCHALES (CHLORIDE)

EDTA TITRATION METHOD (CALCIUM)

Question 9

Measures amount of light BLOCKED

Answer 9

TURBIDIMETRY

Question 10

On what tests is turbidimetry used

Answer 10

protein measurements
detect bacterial growth in broth cultures
broth anti microbial tests
detect clot formation

Question 11

determines amount of light SCATTERED

Answer 11

NEPHELOMETRY

Question 12

In nephelometry, light is measures at what angle?

Answer 12

15-90 degrees

Question 13

in turbidimetry, light transmitted is ____________ to concentration

Answer 13

INVERSELY PROPORTIONAL

Question 14

in nephelometry, detector output is _______________ to concentration

Answer 14

DIRECTLY PROPORTIONAL

Question 15

used for measurement of antigen-antibody complex

Answer 15

NEPHELOMETRY

Question 16

what are the light sources of nephelometry

Answer 16

mercury-arc lamp, tungsten-filament lamp, light-emitting diode, and a laser

Question 17

what type of scatter includes particle size larger than the wavelength of the light source

Answer 17

MIE SCATTER

Question 18

type of scatter wherein the particles that are smaller will scatter light in many directions

Answer 18

RALEIGH SCATTER

Question 19

What type of scatter occurs when cell size is larger than transmitted light (MIE SCATTER)

Answer 19

FORWARD SCATTER

Question 20

What type of scatter occurs when there is granularity and nuclear complexity in cells?

Answer 20

SIDE SCATTER

Question 21

migration of charged particles. used to separate and identify proteins

Answer 21

ELECTROPHORESIS

Question 22

Buffer for alkaline electrophoresis

Answer 22

BARBITAL (8.6)

Question 23

Buffer for acid electrophoresis

Answer 23

CITRATE (3-6.2)

Question 24

supporting media that can separate by molecular size

Answer 24

Cellulose acetate

Question 25

supporting media that can separate by electric charge

Answer 25

agarose gel

Question 26

supporting media that can separate on the basis of charge and molecular size

Answer 26

polyacrylamide gel

Question 27

Single most widespread clinical application of serum electrophoresis

Answer 27

DETECTION OF MONOCLONAL GAMMOPATHY

Question 29

Technique where solutes are separated for identification based on physical differences that allow their differential distribution between a mobile phase and a stationary phase

Answer 29

CHROMATOGRAPHY

Question 30

2 forms of chromatography

Answer 30

planar and column

Question 31

used for fraction of sugar and amino acid

Answer 31

PAPER CHROMATOGRAPHY

Question 32

sorbent used in the stationary phase of paper chromatography

Answer 32

Whatman paper

Question 33

semiquantitative drug screening test

Answer 33

THIN LAYER CHROMATOGRAPHY

Question 34

extraction if drug is ____ dependent

Answer 34

pH dependent

Question 35

biological sample used for TDM

Answer 35

SERUM/PLASMA

Question 36

biological sample used for prohibited drugs

Answer 36

URINE

Question 37

relative distance of migration from the point of application

Answer 37

RETENTION FACTOR VALUE

Question 38

used for separation of steroids, barbiturates, blood, alcohol and lipids

Answer 38

GAS CHROMATOGRAPHY

Question 39

useful for compounds that are naturally volatile

Answer 39

GAS CHROMATOGRAPHY

Question 40

based on the fragmentation and ionization of molecules

Answer 40

MASS SPECTROSCOPY

Question 41

GOLD STANDARD for drug testing

Answer 41

GAS CHROMATOGRAPHY

Question 42

can detect 20 inborn error of metabolism from a single blood spot

Answer 42

TANDEM MASS SPECTROSCOPY (MS/MS)

Question 43

identifies proteins of bacteria/fungi

Answer 43

MALDI-TOF MS