cloning Flashcards
(6 cards)
1
Q
micropagation
A
used to clone plants
2
Q
micropropagation method
A
- plant with desirable characteristics is selected to be cloned. Explants are taken from the tips of the stem and the sideshoots of this plant.
- explants are sterilised to kill any microorganisms
- explants are grown in vitro and placed in a petri dish containing a nutrient medium - contains growth hormones
- cells in explants divide and grow into a small plant.
- small plants are taken out of medium, planted in soil and put into greenhouses.
- will be genetically identical to original plant.
3
Q
cloning an adult mammal ?
A
by transplanting a cell nucleus
4
Q
cloning adult mammal method
A
- nucleus of sheep’s egg cell removed creating an enucleated cell
- take a diploid nucleus from a different sheep and insert it in its place
- cell stimulated with electric shock so it started dividing by mitosis
- dividing cell is implanted into uterus of another sheep called surrogate mother
5
Q
cloning transgenic animals used for?
A
producing human proteins
6
Q
how does cloning transgenic animals work ?
A
- by transferring human genes into cells of animals such as cows and sheep then can produce human proteins in milk.
- can produce human antibodies that are used in arthritis amd candle and multiple sclerosis