CMV Flashcards
What responses are required for LCMV?
CD8 and neutralising antibodie.
CD8 responses required for at least 3 apitopes to avoid escape variants.
How does clone 13 of LCMV escape immunity? What helps to maintain good functional CD8 responses?
by inducing CTL exhuastion.
Good high CD4 responses hselp to maintain active CTLs.
As well as PD-1 what other upregulated molecule is exhausted?
LAG-3 and CD39 (along with CD73)
HOw to attach HLA to fluoresecent molecules in tetraemres/
biotyinylated HLA with fluorescently lablled extravidin.
4 outcomes of HCV infection?
acut disease (normally asymtpmotci)
infection can be:
resolved
persist in most cases- progressive disease, or mild disease.
What kind of T cell response is thought to be good for clearing HCV?
CD4 and CD8 were important, needed to be a broad and high magnitude CD4 and CD8 T cell response.
Involved IFn-y production from CD8 specific T cells.
What kind of T cell response exists after HCV perstistnec and which is associated with escape?
mostly monospecific, exhuasted phenotype, but no escape from CD4 but escape from CD8.
What phenotype marker do MAIT cells in the liver have? and what TCR?
Va7.2 MAIT cells
CD161 in the liver, give very fast IL-17 responses (as well as IFN-Y).
What are MAIT cells resticted by?
Va7.2 with Ja33/12/20 and restricted to MR1.
what is presented on MR1 for MAIT cell recognition?
riboflavin, 5-OP-RU
Transcription factors of MAIT cells?
RORyt adn PLZF.
may also sense viruses.
What three viruses can activate MATI cells in vitro?
dengue, HCV and infulenza- can be independent of MR1 but rapid.
Three cytokines particuarly important in activation of MATI cells?
Il-12 Il15 and Il18.
Are MAIT cells protective in flu in mice and are they doing killing?
protective but not thorugh to be viral elimination.
Early alert system and infiltiration of T cell and macrophages is faster (overlap with ILC1 roles)
What mechanism might Ad vectors use to stimulate MAITs?
trigger pDC IFN-a. This stimulates monocyte production of IL-18 and TNF-a.
IFn-a+ TNF-a + IL-18 stimulate MAITS.
INF-a.TNF>IL-18.
