Coagulation testing

Question 1

Electro-mechanical measurement

Answer 1

probe detects a fibrin clot by moving back and forth

Question 2

An under filled tube will

Answer 2

prolong results

Question 3

Traumatic draw/ hemolysis

Answer 3

APTTs shortened

Question 4

Clotted sample

Answer 4

prolongs results, factors are used up

Question 5

Heparin contamination

Answer 5

prolongs results

Question 6

Normal factor activity

Answer 6

> =50%

Question 7

Low factor activity

Answer 7

25-30%

Question 8

Protime (PT)

Answer 8

VII, X, V, II, I, Extrinsic and common pathways

Question 9

PT reagent

Answer 9

Tissue thromboplastin and calcium

Question 10

PT reference range

Answer 10

11.0-13.4 seconds

Question 11

Activated Partial Thromboplastin Time (APTT)

Answer 11

Prekallikrein, HMWK, XII, XI, IX, VIII, X, II, I, Intrinsic and common pathways

Question 12

APTT reagent

Answer 12

Platelet phospholipid (PL/PF3) substitute, in vitro surface activator such as kaolin or silica

Question 13

APTT reference range

Answer 13

22.0-37.0 seconds

Question 14

Thrombin Time

Answer 14

Measures tine it takes for thrombin to convert fibrinogen to fibrin, measures factor I.

Question 15

Causes of a prolonged thrombin time

Answer 15

low fibrinogen, heparin, FDPS, dysfibrinogemia

Question 16

Fibrinogen level

Answer 16

Quantative test used to measure fibrinogen in plasma, uses thrombin time and standard curve

Question 17

Mixing study

Answer 17

Differentiate factor deficiency from circulation inhibitor, 1:1 mix of patient with normal plasma that contains all factors

Question 18

Corrected mixing study equals

Answer 18

factor deficiency

Question 19

Uncorrected mixing study equals

Answer 19

circulating inhibitor

Question 20

Factor assay

Answer 20

measures factor activity, done after mixing study to determine which factor is deficient

Question 21

Urea Solubility test/5 M urea test

Answer 21

only test available to measure XIII, normally a clot dissolves in >24hrs

Question 22

FDP and D-dimer tests are

Answer 22

Immunoassays

Question 24

D-Dimer test

Answer 24

Detects D-dimers from specifically the degradation of cross-linked fibrin (DXD)

Question 25

A positive D-dimer test indicates

Answer 25

DIC or pulmonary embolism

Question 26

PLT function assay (PFA)

Answer 26

in vitro test that measures platelet plug formation, prolonged by abnormal plt function and counts below 100k

Question 27

PLT aggregation test

Answer 27

measures aggregation with aggregating agents, ADP, thrombin, collagen, epinphrine, arachidonic acid, and ristocetin, normal plts will aggregate.