Coagulaton Tests and Fibrinolysis

Question 1

Manual techniques

Answer 1

Tilt tube, wire loop

Question 2

REQUIRES GENTLE TILTING OF THE TUBE BACK AND FORTH AT THE RATE OF ABOUT ONCE PER SECOND UNTIL A FIBRIN WEB IS FORMED.

Answer 2

TILT TUBE METHOD

Question 3

EMPLOYS THE USE OF A WIRE LOOP THAT IS PASSED THROUGH THE MIXTURE AT THE RATE OF TWO SWEEPS PER SECOND UNTIL A FORMED
CLOT ADHERES TO THE LOOP.

Answer 3

NICHROME WIRE LOOP TECHNIQUE

Question 4

Automated techniques

Answer 4

MOVING PROBE, OPTICAL DENSITY

Question 5

A CLOT IS DETECTED EITHER BY USE OF A ______ IMMERSED IN THE MIXTURE THAT IS TRIGGERED BY CLOT FORMATION

Answer 5

MOVING PROBE

Question 6

A CLOT IS DETECTED EITHER BY USE OF A MOVING PROBE OR BY THE CHANGE IN ____ OF THE MIXTURE WHEN A CLOT FORMS.

Answer 6

OPTICAL DENSITY

Question 7

SCREEN SECONDARY HEMOSTASIS
(INTRINSIC AND COMMON PATHWAY)

Answer 7

LEE AND WHITE METHOD

Question 8

LEE AND WHITE METHOD principle

Answer 8

EXPOSE BLOOD TO A NEGATIVELY CHARGED SURFACE

Question 9

NV FOR LEE AND WHITE METHOD

Answer 9

5-15 min

Question 10

The surface of the glass tube initiates the Clotting process. This test is sensitive to the factors involved in the intrinsic pathway

Answer 10

Clotting Time - Slide Method
(Intrinsic)

Question 11

The expected range for Slide Method Clotting Time is

Answer 11

4-10 min.

Question 12

LABORATORY SCREENING

Answer 12

• PROTHROMBIN TIME
• ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)

Question 13

IS A USEFUL SCREENING PROCEDURE FOR THE EXTRINSIC AND COMMON PATHWAY.

Answer 13

PROTHROMBIN TIME

Question 14

Sample for PROTHROMBIN TIME

Answer 14

CITRATED PLASMA

Question 15

REAGENT: PROTHROMBIN TIME

Answer 15

TISSUE THROMBOPLASTIN
0.025 MOL/L SOLUTION OF CALCIUM CHLORIDE.

Question 16

NORMAL RANGE: PROTHROMBIN TIME

Answer 16

10-15 SEC

Question 17

MEASURES ALL FACTORS IN THE INTRINSIC
AND COMMON PATHWAY EXCEPT FACTOR XIII (fibrin stabilizing) AND PLATELETS.

Answer 17

ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)

Question 18

ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) SAMPLE:

Answer 18

CITRATED PLASMA

Question 19

ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) REAGENT:

Answer 19

PARTIAL THROMBOPLASTIN, CALCIUM CHLORIDE, ACTIVATORS

Question 20

ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) ACTIVATORS:

Answer 20

SILICA, KAOLIN, ELLAGIC ACID, CELITE

Question 21

ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) NORMAL RANGE:

Answer 21

28-46 SEC

Question 22

MEASURES THE AVAILABILITY OF FUNCTIONAL FIBRINOGEN OR DECREASED AMOUNT (75-100 MG/DL). AFFECTED BY HEPARIN.

Answer 22

THROMBIN TIME (TT)

Question 23

THROMBIN TIME (TT) PRINCIPLE:

Answer 23

MEASURED THROMBIN IS ADDED TO PLASMA AND TIME FOR FIBRIN CLOT
FORMATION IS RECORDED.

Question 24

FIBRINOGEN DECREASED AMOUNT

Answer 24

(75-100 MG/DL).

Question 25

THROMBIN TIME (TT)

Answer 25

10-14 SEC (BROWN)

Question 26

Thrombin time is affected by

Answer 26

Heparin

Question 27

SAME AS THROMBIN TIME BUT NOT AFFECTED BY HEPARIN.

Answer 27

REPTILASE TIME

Question 28

IS AN ENZYME FOUND IN THE VENOM OF THE BOTHROPS ATROX SNAKE THAT CAN CONVERT FIBRINOGEN O FIBRIN.

Answer 28

REPTILASE (ATROXIN)

Question 29

SNAKE THAT CAN CONVERT FIBRINOGEN O FIBRIN.

Answer 29

BOTHROPS ATROX SNAKE

Question 30

TIME IS RECORDED AS CLOT IS FORMED AFTER REPTILASE IS ADDED TO PLASMA.

Answer 30

REPTILASE TIME

Question 31

REPTILASE TIME NV

Answer 31

10-15 SEC

Question 32

USED TO DETECT DEFICIENCIES OF THE COMMON PATHWAY (FACTORS X, V, II AND I)

Answer 32

STYPVEN TIME (RUSSELL'S VIPER VENOM TIME)

Question 33

IS A THROMBOPLASTIN LIKE SUBSTANCE THAT ACTIVATES FACTOR X.

Answer 33

STYPVEN

Question 34

STYPVEN TIME (RUSSELL'S VIPER VENOM TIME) NV:

Answer 34

6-10 SEC

Question 35

COMMON PATHWAY FACTORS

Answer 35

(FACTORS X, V, |I AND I)

Question 36

DETECTS FACTOR XIII DEFICIENCY

Answer 36

DUCKERT'S TEST/ 5M UREA SOLUBILITY TEST

Question 37

IN DUCKERT'S TEST/ 5M UREA SOLUBILITY TEST CLOT FORMED AFTER COAGULATION IS ADDED WITH ___

Answer 37

5M UREA

Question 38

SUBSTITUTE FOR 5M UREA

Answer 38

• 1% MONOCHLORACETIC ACID • 2% ACETIC ACID

Question 39

Last phase of coagulation cascade

Answer 39

Fibrinolysis

Question 40

IT IS THE SYSTEMIC, ACCELERATING HYDROLYSIS OF FIBRIN BY PLASMIN

Answer 40

FIBRINOLYSIS

Question 41

FIBRINOLYSIS IS THE SYSTEMIC, ACCELERATING HYDROLYSIS OF FIBRIN BY

Answer 41

PLASMIN

Question 42

Functions of plasmin

Answer 42

-Promotes Cell Migration -Remodels ECM -Degrades Fibrin

Question 43

'Other Coagulation tests

Answer 43

- Thrombin time - Reptilase time - Stypven Time (Russell's Viper Venom Time) -Duckerts test / 5 M urea solubility test

Question 44

SERINE PROTEASE ZYMOGEN;

Answer 44

PLASMINOGEN

Question 45

PLASMINOGEN ACTIVE FORM WHICH DIGESTS FIBRIN AND FIBRINOGEN

Answer 45

PLASMIN

Question 46

SECRETED BY ENDOTHELIUM AND ACTIVATES PLASMINOGEN

Answer 46

TISSUE PLASMINOGEN ACTIVATOR (TPA)

Question 47

SECRETED BY KIDNEYS AND ACTIVATES PLASMINOGEN

Answer 47

TISSUE PLASMINOGEN ACTIVATOR (TPA)

Question 48

SECRETED BY KIDNEYS AND ACTIVATES PLASMINOGEN

Answer 48

UROKINASE

Question 49

FROM ENDOTHELIUM, INHIBITS ТРА

Answer 49

PLASMNIOGEN ACTIVATOR INHIBITOR 1 (PAI-1)

Question 50

INHIBITS PLASMIN

Answer 50

A2-ANTIPLASMIN

Question 51

SUPPRESSES FIBRINOLYSIS

Answer 51

THROMBIN ACTIVATABLE FIBRINOLYSIS INHIBITOR

Question 52

THE FIBRIN FORMED IS COMPOSED OF TWO DOMAINS:

Answer 52

D AND E

Question 53

2. PLASMINOGEN IN PLASMA IS ACTIVATED BY TPA AND UROKINASE INTO ACTIVE ENZYME ____

Answer 53

PLASMIN

Question 54

3. PLASMIN, THEN CLEAVES THE PEPTIDE BONDS ON THE ___ AND ___ AMINO ACID THAT CONNECTS THE TWO DOMAINS.

Answer 54

ARGININE AND LYSINE

Question 55

4. THE FIBRIN DIGESTED WILL THEN FORM THE FIBRIN DEGRADATION PRODUCTS (FDPS) NAMELY:

Answer 55

FRAGMENT X (D-E-D), FRAGMENT Y (D-E), FRAGMENT D, FRAGMENT E, AND FRAGMENT D-D (D-DIMER).