Collisional activation

Question 1

Collisional activation

Answer 1

Give molecular ion more energy to fragment it (ES or MALDI)

Question 2

How are molecular ions collisionally activated?

Answer 2

Exposure to collision gas as they pass through a collision chamber located between 2 analysers

Question 3

How does MS/MS work

Answer 3

1st analyser selects ions produced in source and collisionally activates them. 2nd analyser separates fragment ions and any non-fragmented molecular ions

Question 4

How does triple quadrupole work?

Answer 4

Molecular ions selected in 1st (m/z), fragmented in 2nd (collision gas), fragments analysed in 3rd. - Robust, reliable, cheap

Question 5

Q-TOF

Answer 5

Quadrupole allows all ions through, TOF = mass analyser (pulses of ions accelerated into TOF by “pusher”)

Question 6

Q-TOF/TOF

Answer 6

Quadrupole allows selected ions through collision cell (cont. collision gas). TOF = mass analyser