Proteomics Flashcards
1
Q
Proteomics
A
Analysis of protein
2
Q
What is a widely used technique for proteomics
A
2D gel electrophoresis (2DGE)
3
Q
Why are gels used
A
Quick, inexpensive, good resolving technique, good visual representation
4
Q
Detection in 2DGE
A
Coomassie blue, silver staining, radiolabelling, fluorescent stain
5
Q
How does MS work on gel
A
Identifies the spots
6
Q
What is the proteomics technique used to identify proteins called
A
Mapping or fingerprinting. Can be MS or MS/MS
7
Q
What can nanoLC-ES-MS/MS identify
A
PTMs
8
Q
What is SDS page biased against?
A
Low abundance proteins, integral membrane proteins, proteins with extremes of pI, proteins with extremes of MW
9
Q
Shotgun proteomics
A
Attempts to analyse all proteins in a sample
10
Q
MudPIT
A
Multidimensional Protein Identification Technology
