Colony Morphology Flashcards
(45 cards)
refers to the inoculation of the clinical specimen onto laboratory media
Primary plating
Generally, microbiologists observe the colony
morphology of organisms isolated on primary culture
after how many hours?
18 to 24 hours
interpretation of primary cultures
Plate reading
laboratory media that contains added agents to inhibit the growth of certain species
selective media
permits the differentiation of bacterial strains based on colony morphology based on the ability of some bacteria, and not others
differential media
what agar support the growth of fastidious and non-fastidious organisms?
SBA and CHOC agar
what is the term called for hard to grown and requires additional growth factors?
fastidious organisms
it has the key ingredients of intact sheep red blood cells, vitamin K, and yeast extracted
anaerobic blood agar
- Nutritious medium
- for the isolation
and - subculturing of
obligate anaerobic
bacteria
anaerobic blood agar
- Hemolyzed
sheep red blood
cells - supplemented
with Hgb and
NAD
Chocolate agar
- Primary plating
and - subculturing of
fastidious
bacteria, - e.g., Haemophilus
and pathogenic
Neisseria spp
Chocolate agar
- carbohydrates
lactose, - salicin, and
- sucrose; and
- bile salts to
inhibit growth of
gram-positive
bacteria and
many
nonpathogenic
enteric bacteria
Hektoen energetic agar
- selective and
- differential
medium - for the primary
plating of stool
specimens - to aid in the
recovery of
intestinal
pathogens, - e.g., Salmonella
Hektoen enteric agar
- Lactose and
- bile salts,
- the low
concentration of
bile salts inhibits
gram-positive
bacteria but - permits the
growth of many
gram-negative
bacteria
MacConkey agar
- selective and
- differential
medium - for the isolation
of gram-negative
bacteria; - used for primary
plating and
subculturing
MacConkey agar
- Chocolate agar
base - containing the
antimicrobial
agents: - vancomycin,
colistin,
trimethoprim, and
nystatin
Modified thayer- martin agar
- selective primary
plating medium - for recovery of
Neisseria
gonorrhoeae and
Neisseria
meningitidis
Modified thayer - martin agar
- Dextrose
(glucose), - antimicrobials
can be added to
inhibit bacteria
Sabouraud dextrose agar
- Primary plating
and subculturing
of fungi
Sabouraud dextrose agar
- Intact sheep red
blood cells
Sheep blood agar
- Primary plating
and subculturing
of most bacterial
isolates
Sheep blood agar
provides additional nutrients to support
highly fastidious organisms
CHOC agar
a process in which bacteria selected for
further testing will be inoculated to other plates
Subculturing
supports most gram-negative rods,
especially Enterobacteriaceae
MAC agar
