Comparision between light microscope & TEM (C2)

Question 1

source of radiation

Answer 1

LM-> Visible light rays

EM -> electron beam

Question 2

resolving power

Answer 2

LM-> 200nm

EM -> 1nm (for TEM)

Question 3

magnification

Answer 3

LM-> 1500x

EM -> 500000x

Question 4

Condenser lens

Answer 4

LM-> glass lenses

EM -> electromagnet

Question 5

magnetic fields

Answer 5

LM-> unaffected

EM -> affected

Question 6

biological specimen

Answer 6

LM-> living or dead

EM -> dead, very thin and dehydrated sections are needed

Question 7

stains

Answer 7

LM-> may be stained or unstained

EM -> treated with solutions of heavy metals, natural colour cannot be seen

Question 8

image

Answer 8

LM-> enlarge image which can be seen by human eye, less distortion of material
EM -> image formed on fluorescent screen or electron micrographs are produced, electrons are nt visible to the human eye, complex preparation may cause distortion of material

Question 9

depth of field

Answer 9

LM-> restricted

EM -> greater depth of field

Question 10

size of microscope

Answer 10

LM-> small and portable

EM -> large and must be operated in special rooms

Question 11

cost

Answer 11

LM-> relatively cheap

EM -> expensive

Question 12

preparation of specimen

Answer 12

LM-> quick and simple

EM -> time consuming and requires expertise to operate the complex equipment