Control of Microbial Growth (Lab) Flashcards
(22 cards)
A process of destroying all forms of microbial life including the endospores.
Sterilization
most resistant forms of life
endospores
What is the apparatus used, time needed, temperature required, and examples of materials used in steam under pressure?
Autoclave
15-20 minutes
121 degrees Celsius
Bacteriologic media
What is the apparatus used, time needed, temperature required, and examples of materials used in free flowing steam/Tyndallization/intermittent?
Arnold sterilizer
30 minutes for 3 consecutive days with incubation period in between sterilization
100 degrees Celsius
Culture media that cannot withstand autoclave temp.
What is the apparatus used, time needed, temperature required, and examples of materials used in boiling?
Boiler
10 minutes
100 degrees Celsius
Water, food, and utensils, dental supplies
What is the apparatus used, time needed, temperature required, and examples of materials used in inspissation?
Inspissator
2 hours for 3 successive days
75-80 degrees Celsius
High protein media
What is the apparatus used, time needed, temperature required, and examples of materials used in pasteurization?
Pasteurizer
30 minutes, 15 seconds, less than a second
63 degrees Celsius, 72 degrees Celsius, 141 degrees Celsius
Alcoholic beverages, milk, food
heat that kills microbes by coagulation of proteins by the breakage of hydrogen bonds that hold the proteins in three dimensional structures
moist heat
heat that kills by oxidation effects
dry heat
What are the 3 types of Dry heat?
Direct flaming
incineration
hot air sterilization
What is the apparatus used, time needed, temperature required, and examples of materials used in direct flaming?
Bunsen burner
until red glow/hot
N/A
inoculation instruments, tip of forceps
What is the apparatus used, time needed, temperature required, and examples of materials used in incineration?
Incinerator
N/A
870-980 degrees Celsius
Hazardous materials (paper cups, animal carcasses)
What is the apparatus used, time needed, temperature required, and examples of materials used in hot air sterilization?
Oven
2 hours
160-180 degrees Celsius
petri dishes
Give 3 examples of heat sensitive materials
culture media, enzymes, serum, vaccines, antibiotics
It is the passage of liquid or gas through a screen-like material, pores are small enough to retain microorganisms. It is also used to sterilize heat sensitive materials.
Filtration
What are the 4 types of filter and their composition?
Berkefeld - diatomaceous earth
Seitz - asbestos pad
Chamberland Pasteur - unglazed porcelain
Morton - sintered glass
This is a system of laminar flow filter which is used extensively to provide dust-free and bacteria-free air
High efficiency particulate air (HEPA)
What is the principal effect of ionizing radiation? and Why?
Ionization of water because it forms highly reactive hydroxyl radicals
Give an example of a non-ionizing radiation
UV light
What type of radiation is used to disinfect vaccines and other medical products?
Non-ionizing radiation
What is the most important precaution in preventing nosocomial infections?
Handwashing
How long should we handwash to remove most transient organisms?
30 seconds
