CSE # 4

Question 1

what are the biochemical tests for Staphylococcus?

Answer 1

Thioglycollate = oxygen requirement, facultative anaerobe , growth throughout
catalase= bubbling
glucose = fermentation of glucose, purple to yellow
coagulase = presentence of the enzyme coagulase

Question 2

What are the biochemical test for Streptococcus?

Answer 2

CAMP test = group B, super hemolytic flow
bile esculin = group D, black it positive
6.5% NaCl = group D growth w/ yellow
catalase = not bubbling

Question 3

gram stain report for Staph, Strep, and Entero?

Answer 3

staph = gram Positive cocci clusters
strep = gram positive cocci chains
Entero = gram negative rod

Question 4

what is primary vs opportunistic pathogens?

Answer 4

primary - known to cause infection
opportunistic - has the ability to cause infections in certain conditions

Question 5

where are staph strep and entero, found?

Answer 5

staph = skin mucus membranes
strep =
entero = medical devices, intestines

Question 6

fermentation broths? indicator?

Answer 6

glucose, fructose, sucrose
- fermentation of sugars
- indicator is Phenol red
+ turns yellow

Question 7

Methyl red Test , Indicator?

Answer 7

tests for mixed acids
- fermentation of glucose - produces = lactic, acetic, and formic acid
Indicator is Methyl red (pH)
+ turns red

Question 8

Voges-Proskauer test

Answer 8

fermentation of glucose and acetone
- product of butanediol fermentation
reagent reacts with acetone in oxidation reduction
+ turns reddish color

Question 9

Indole Tests indicator?

Answer 9

ability to degrade amino acid, tryptophan into indole
- kovacs reagent
+ turns pink with layer on top

Question 10

Citrate test indicator?

Answer 10

tests to see if citrate is main carbon source
- creates alkaline products (pH)
- indicator is bromothymol blue
+ slant turns blue

Question 11

Urease test indicator?

Answer 11

ability to hydrolyze urea to ammona and CO2
UTI produce urease
- phenol red
+ hot pink

Question 12

Phenylalanine deaminase test

Answer 12

presence of enzyme phenylalanine deaminase
+ green layer on slant

Question 13

Motility test?

Answer 13

detects ability to move presence of flagella or flagellum TTC indicates active metabolism

Question 14

What does XLD select for ?

Answer 14

Gram negative
- differential providencia and salmonella from enter. bacteria
- fermentation of xylose
-hydrogen sulfide production

Question 15

What are the 3 bacteria that can grow on XLD?

Answer 15

salmonella = red w/ black centers (food)
shigella = red colonies
coliforms = yellow to orange bacteria (gut)

Question 16

What are the 3 bacterias that can grow on EMB?

Answer 16

• fermentation of glucose
  non lactose fermenters = pink
  weak lactose fermenters = purple
  stong lactose fermenters = metallic green

Question 17

Alpha hemolysis
Beta hemolysis
Gamma hemolysis

Answer 17

alpha - partial destruction of RBC with no use of hemoglobin
Beta - complete destruction of RBC and hemoglobulin
Gamma - lack of hemolysis