Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Mycology & Virology > Culture Media > Flashcards

Culture Media Flashcards

(60 cards)

Start Studying
1
Q

Used for the cultivation of ascosporogenous yeasts such as Saccharomyces cerevisiae.

A

Acetate ascopore agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

better sporulation medium than sodium acetate.

A

potassium acetate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Ascopores from acetate ascopore agar are stained with

A

Kinyoun carbol fuschin acid fast stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Selective and differential medium used for the isolation of Cryptococcus spp

A

Birdseed, Niger seed agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

C. neoformans and C. gattii are is unique in that they produce the enzyme

A

phenol oxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

The breakdown of substrate (Guizotia abyssinica seeds or niger seeds) produces this, imparting a tan to brown pigmentation to the colonies

A

melanin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

selective ageint in birdseed agar that inhibits bacterian and other fungi

A

chloramphenicol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

enhances melanization of C. neoformans in birdseed agar

A

creatinine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Selective and differential medium used for the isolation and differentiation of Candida spp.

A

Bismuth sulfite-glucose-glycine yeast (BiGGY) agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

nutritive base of BiGGY

A

peptone
glucose
yeast extract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Candida reduce ____ to _____

A

bismuth sulfite, bismuth sulfide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

inhibitor of bacterial growth in BiGGY

A

bismuth sulfite

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

appearance of C. albicans in BiGGY

A

black, no pigment diffusion, no sheen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

appearance of C. tropicalis in BiGGY

A

dark brown colonies with black center
black pigment diffusion
sheen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

used for the cultivation and isolation of all fungi including fastidious dimorphic fungi.

A

Brain Heart Infusion Agar with 10% sheep red cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

inhibitors present in BHI

A

chloramphenicol and gentamicin (bacteria)
cycloheximide (saprobes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Adaptation of birdseed agar.

A

caffeic agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

the biologically active chemical in niger seed that causes the yeast colony of C. neoformans to turn brown

A

caffeic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Differential medium to distinguish C. neoformans from C. gattii.

A

Canavanine-glycine-bromthymol blue agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

incubation time and temperature of Cryptococcus on Canavanine-glycine-bromthymol blue agar

A

30oC for 1 to 5 days.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

turns Canavanine-glycine-bromthymol blue agar cobalt blue

A

C. gattii (serotypes B and C)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

leave the Canavanine-glycine-bromthymol blue agar medium greenish yellow.

A

C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Chromogenic, differential and selective medium for the isolation of clinically important yeasts.

A

CHROMagar (BD BBL; BD Diagnostics Systems)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Nutritive base of CHROMagar

A

peptone and glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
inhibits bacterial growth in CHROMagar
Chloramphenicol
26
CHROMagar with fluconazole is available for this fluconazole-resistant fungi
C. krusei
27
More sensitive than SDA and helpful in identifying mixed cultures of yeasts
CHROMagar
28
CHROMagar enhances the rapid assimilation of _____ by C. glabrata
trehalose
29
colonies on CHROMagar should be evaluated at
48 h
30
used for Presumptive identification of Cryptococcus, Trichosporon, Rhodotorula spp.
Christensen’s urea agar
31
Christensen’s urea agar contains
urea and phenol red serving as indicator
32
facilitates separation of certain dermatophytes viz., T. mentagrophytes and T. rubrum.
Urea hydrolysis
33
Used for the cultivation and differentiation of T. mentagrophytes from T. rubrum on the basis of pigment production.
Cornmeal agar with 1% dextrose
34
used for the differentiation of Candida species on the basis of morphological characteristics.
Cornmeal agar with Tween 80
35
specifically incorporated in lieu of dextrose for the demonstration of pseudohyphal, chlamydospores, and arthrospores formation.
Tween 80 (surfactant)
36
placing a cover slip over the yeast inoculum to create a microaerophilic environment
Dalmau method
37
Used for the differentiation of Aspergillus spp.
Czapek-Dox agar
38
sole carbon source in Czapek-Dox agar
sucrose
39
the sole nitrogen source in Czapek-Dox
sodium nitrate
40
Any bacteria or fungi that can use sodium nitrate as a nitrogen source can grow on this medium.
Czapek-Dox agar
41
Screening medium for the recovery, selection, and differentiation of dermatophytes from keratinous specimens.
Dermatophyte test medium
42
inhibits saprophytic moulds
cycloheximide
43
inhibits Gram-negative bacteria.
cycloheximide
44
Morphology and microscopic characteristics are easily identified, but pigmentation cannot be discerned because of the presence of phenol red indicator.
Dermatophyte test medium (DTM)
45
Dermatophyte test medium (DTM) is color _____ and turns ______ with dermatophyte growth
yellow, red
46
Used for the isolation and growth of lipodependent Malassezia spp.
Leeming and Notman medium
47
Components of Leeming and Notman medium
ox bile glycerol monostearate glycerol Tween 80 cow’s milk (whole fat)
48
may serve as an alternative to SDA
Leeming and Notman medium
49
Selective general-purpose medium used for the isolation of fungi from contaminated specimen.
Littman oxgall agar
50
selective agents inhibiting bacteria in Litmann oxgall agar
Crystal violet and streptomycin
51
restricts the spreading of fungal colonies in Littman oxgall agar
oxgall
52
selective medium specifically formulated for the isolation of dermatophytes but also for the isolation of other pathogenic fungi from specimens contaminated with saprophytic fungi and bacteria.
Mycobiotic or Mycosel agar
53
inhibitor in Mycobiotic or Mycosel agar
cycloheximide and chloramphenicol
54
Inhibited fungi in Mycobiotic or Mycosel agar
Candida and Aspergillus spp., mucoraceous fungi C. neoformans.
55
Used to stimulate conidium production by fungi and stimulates pigment production in some dermatophytes.
Potato Dextrose
56
Commonly used with the slide culture technique to view morphological characteristics.
Potato Dextrose
57
lowers the pH of potato dextrose medium thereby inhibiting bacterial growth
tartaric acid
58
most common culture media in mycology
Sabouraud dextrose agar (SDA)
59
SDA consists of
pancreatic digest of casein peptic digest of animal tissue dextrose at 4% concentration buffered to a pH of 5.6.
60
the selective components of SDA are
1. Chloramphenicol 2. Cycloheximide 3. Gentamicin 4. Ciprofloxacin 5. Penicillin and(or) streptomycin

Mycology & Virology (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions