Culture Media and its Preparation page 4 Flashcards
(20 cards)
media can be obtained premixed, in an often dehydrated and powdered state
preprepared medium
preprepared media to which additional growth factors have been added
enriched media
example of enriched medium that is made from complex medium to which blood has been added
blood agar
employed for growing obligate anaerobes
reducing medium
contains chemicals that depletes molecular oxygen
reducing medium
suppress the growth of some microorganisms while allowing the growth of others
selective medium
examples of selective media
mannitol salts agar
MacConkey agar
eosin-methylene blue agar
phenylethyl alcohol agar
selects against non-skin flora
mannitol salts agar
selects against gram positives
MacConkey agar and eosin-methylene blue agar
selects against gram negatives
phenylethyl alcohol agar
allows the growth of more than one microorganism of interest but with morphologically distinguishable colonies
differential media
examples of differential media
mannitol salts agar
blood agar
MacConkey agar
eosin-methylene blue agar
mannitol fermentation color
yellow
mannitol fermentation = yellow
mannitol salts agar
various kinds of hemolysis
blood agar
lactose fermentation color
yellow
lactose fermentation=yellow
MacConkey agar
various kinds of differentiation
eosin-methylene blue agar
used to increase the relative concentration of certain microorganisms in the culture prior to plating on solid selective medium
enrichment culture
True or False: Enrichment culture is typically used as broth medium
true
