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CLINICAL BACTERIOLOGY > Culture Media - Function > Flashcards

Culture Media - Function Flashcards

Culture media according to function (86 cards)

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1
Q

Differential medium used to distinguish Escherichia coli from Shigella spp.

A

Acetate agar

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2
Q

Vibrio and Aeromonas spp. from stool specimens

A

Alkaline Peptone Water

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3
Q

For Mycobacterium spp.

A

American Trudeau Society Medium

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4
Q

Selective differential agar used for the isolation and identification of members of the Bacteroides fragilis group

A

Bacteroides bile esculin (BBE) agar

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5
Q

Group D streptococci and the Enterococci and Listeria monocytogenes

A

Bile Esculin Agar

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6
Q

Selective medium for the isolation of Salmonella spp.

A

Bismuth Sulfite Agar

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7
Q

Enrichment medium for the isolation of moderately fastidious, obligate anaerobes

A

Blood Agar, Anaerobic, Brucella Base, Wadsworth

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8
Q

Enriched medium useful for the isolation of fastidious anaerobes.

A

Blood Agar, Anaerobic, CDC

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9
Q

Selective enrichment medium recommended for the isolation of species of Bacteroides and Prevotella.

A

Blood Agar, Anaerobic, Laked, With Kanamycin, Vancomycin, and Vitamin K

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10
Q

It is useful in the primary isolation of obligate gram-negative anaerobes, particularly Bacteroides spp

A

Blood Agar, Anaerobic, With Kanamycin and Vancomycin

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11
Q

demonstration of β-hemolysis by Haemophilus spp. and Gardnerella vaginalis

A

Blood Agar, Rabbit

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12
Q

Routine medium used to cultivate a wide variety of moderately fastidious bacterial organisms

A

Blood Agar, Sheep

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13
Q

Selective enrichment medium useful in the isolation of Bacteroides, Prevotella, and other obligate anaerobes from specimens containing a mixture of obligate and facultative anaerobes

A

Blood Phenylethyl Alcohol Agar, Anaerobic, CDC

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14
Q

Selective enrichment medium for the isolation of Bordetella pertussis and Bordetella parapertussis

A

Bordet-Gengou Blood Agar

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15
Q

Enriched medium suitable for the cultivation of nonfastidious and moderately fastidious microorganisms.

A

Brain-Heart Infusion Broth

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16
Q

Enrichment medium useful in the isolation of Legionella spp.

A

Buffered Charcoal Yeast Extract Agar

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17
Q

To isolate B. cepacia selectively from respiratory specimens collected from patients with cystic fibrosis

A

Burkholderia cepacia Agar

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18
Q

for the isolation of B. cepacia in respiratory specimens taken from patients with cystic fibrosis

A

Burkholderia cepacia Selective Agar

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19
Q

isolation and cultivation of Campylobacter spp. from stool specimens.

A

Campylobacter Blood Agar (Campy-BA)

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20
Q

for Pseudomonas aeruginosa in specimens with mixed microbiota

A

Cetrimide Agar

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21
Q

enrichment agar that is especially useful in promoting the growth of Haemophilus and other fastidious bacterial species.

A

Chocolate Agar

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22
Q

Selective, differential, chromogenic media that have been developed for the isolation and identification of yeast, Staphylococcus aureus, Escherichia coli O157:H7

A

CHROMagar

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23
Q

useful in differentiating gram-negative enteric bacilli

A

Citrate Agar, Simmons

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24
Q

The basal medium is suitable for cultivation of several aerobic and anaerobic bacterial organisms found in clinical materials. Additionally, it provides an efficient base for preparation of a variety of enrichment agars that support the growth of more fastidious aerobes and anaerobes

A

Columbia Agar With and Without 5% Sheep Blood

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25
Cultivation of anaerobes, especially pathogenic species of Clostridium spp
Cooked Meat (Chopped Meat Glucose) Medium
26
Selective, differential medium useful in the isolation and identification of Clostridium difficile from stool specimens of patients suspected of having antibiotic-associated diarrhea with pseudomembranous colitis.
Cycloserine Cefoxitin Fructose Agar
27
are recommended for the determination of fermentation reactions by fastidious organisms
Cystine Tryptic Agar With Sugar
28
differential medium useful in the identification of fermentative and nonfermentative gram-negative bacteria
Decarboxylase Test Medium (Moeller
29
Differential medium used to detect the production of an active DNase exoenzyme by aerobic bacterial species. Detection of DNA degradation include hydrochloric acid precipitation of undegraded DNA and a color change of an incorporated metachromatic dye, such as toluidine blue or methyl green
Deoxyribonuclease (DNase) Test Agar
30
differentiation of Nocardia spp. from one another and from Streptomyces spp. on the basis of growth and colony morphology. N. asteroides- does not grow or grows poorly with a thin, flaky appearance N. brasiliensis -grows well, forming compact, rounded colonies Streptomyces spp. -produce poor to good growth with a stringy or flaky morphology
Dilute Gelatin Medium (0.4%)
31
Differential medium useful in the detection of lecithinase, lipase, and protease activity
Egg Yolk Agar, CDC Formulation modified McClung Toabe agar,
32
Selective differential medium useful in the isolation and identification of gram-negative enteric bacteria.
Eosin–Methylene Blue agar
33
Differential medium used to determine the ability of an organism to hydrolyze esculin
Esculin Agar
34
Enrichment medium recommended for the detection of leptospiral species in blood, spinal fluid, and urine specimens, as well as possibly contaminated water and other materials
Fletcher Semisolid Medium
35
Differential medium used to determine a bacterium’s ability to produce gelatinase and thereby hydrolyze gelatin.
Gelatin Medium (Nutrient)
36
selective enrichment medium used to enhance the chance of recovering enteric pathogens, such as Salmonella and Shigella spp., from fecal specimens
Gram-Negative Broth
37
Susceptibility testing of Haemophilus isolates is performed on the enriched Haemophilus test medium
Haemophilus Test Medium
38
Selective differential medium used for direct isolation of enteric pathogens from feces and for indirect isolation from selective enrichment broth
Hektoen Enteric Agar
39
Differential broth useful in the identification of Group B streptococci.
Hippurate Broth
40
Detect the production of hydrogen sulfide (H2S) from sulfur containing amino acids
Hydrogen Sulfide, Lead Acetate
41
Determine whether a gram-negative rod is a glucose or lactose fermenter or both for Family Enterobacteriaceae. it resembles triple sugar iron agar (TSI) except that it lacks sucrose. The medium also tests for gas production during carbohydrate fermentation and hydrogen sulfide production
Kligler Iron Agar
42
Isolation of Streptococcus agalactiae, usually from vaginal or rectal swabs obtained from pregnant women who are being screened for carriage of group B streptococci prior to delivery.
Lim Broth (modified Todd-Hewitt broth.)
43
Primarily for the recovery and identification of Corynebacterium diphtheriae. The medium promotes the development o characteristic metachromatic granules that can be detected microscopically with methylene blue stains.
Loeffler Coagulated Serum Slant
44
used to cultivate Mycobacterium spp.
Löwenstein-Jensen Medium
45
Measures three parameters useful in identifying species of Enterobacteriaceae - lysine decarboxylation, lysine deamination, and H2S production.
Lysine Iron Agar
46
Selective, differential, primary plating medium for Enterobacteriaceae and other gram-negative rods in the presence of mixed microbiota and differentiates them into lactose fermenters and non–lactose fermenters A formulation of MAC without crystal violet has been used to help identify Mycobacteria
MacConkey Agar
47
to isolate E. coli O157:H7, which does not ferment sorbitol very rapidly
MacConkey Sorbitol Agar
48
Used in the identification of species of Enterobacteriaceae, particularly Salmonella
Malonate Broth
49
selective and differential primary culture medium useful in the recovery and identification of Staphylococcus aureus (can ferment Mannitol) from specimens containing mixed microbiota
Mannitol Salt Agar
50
useful in distinguishing among members of the family Enterobacteriaceae.
Methyl Red Voges - Proskauer Medium
51
used to cultivate Mycobacterium spp.
Middlebrook 7H10 and 7H11 Agars
52
selective agar is prepared by adding antimicrobial agents to the Middlebrook 7H11 formulation, thereby making the medium more selective for mycobacteria
Mitchison 7H11 Selective Agar
53
selective enrichment medium used for recovering Neisseria gonorrhoeae and Neisseria meningitidis from specimens that have mixed microbiota
Modified Thayer-Martin Agar
54
Test is particularly useful to identify members of the Enterobacteriaceae, in which two genera, Shigella and Klebsiella, are always nonmotile, and certain Yersinia spp. that show motility at room temperature but not at 35° C and the non–glucose fermenting, gram-negative rods can be differentiated based in part on their motility.
Motility Test Medium
55
Transparent medium useful in testing the susceptibility of organisms to antimicrobial agents In addition, sheep blood may be added to the basic formulation to perform susceptibility testing on streptococci, in particular Streptococcus pneumoniae.
Mueller-Hinton Agar
56
detect methicillin-resistant Staphylococcus aureus (MRSA)
Mueller-Hinton Agar With 2% NaCl
57
S. aureus isolates selectively for resistance to oxacillin or nafcillin.
Mueller-Hinton Agar With 4% NaCl and 6 μg Oxacillin
58
Primarily used to isolate N. gonorrhoeae and N. meningitidis from specimens containing mixed normal microbiota.
New York City Medium
59
useful in the recognition of members of Enterobacteriaceae and differentiation of non–glucose-fermenting, gram-negative rods, Neisseria spp., and Moraxella catarrhalis.
Nitrate Reduction Broth
60
General purpose media for growth of non fastidious organisms
Nutrient Agar
61
used to determine whether a gramnegative, non–glucose fermenting rod is oxidative, fermentative, or biochemically inert
Oxidative-Fermentative Medium (Hugh and Leifson Formulation)
62
selective and differential medium used for the isolation of Burkholderia cepacia from the respiratory samples of patients with cystic fibrosis.
Oxidative-Fermentative Polymyxin B–Bacitracin-Lactose Agar
63
Useful for culturing anaerobes.
Peptone–Yeast Extract– Glucose Broth
64
Used to detect an organism’s ability to deaminate phenylalanine. useful for distinguishing Proteus, Providencia, and Morganella spp. from other members of the family Enterobacteriaceae
Phenylalanine Deaminase Agar
65
Selective, differential, enrichment agar useful in isolating Corynebacterium diphtheriae
Potassium Tellurite Blood Agar
65
Used primarily to isolate gram-positive cocci, such as staphylococci and streptococci, from specimens with mixed microbiota.
Phenylethyl Alcohol Agar
66
Isolate Mycoplasma spp. Its name is derived from the original name for Mycoplasma
PPLO Agar
67
enriched and selective for the isolation of Bordetella pertussis and Bordetella parapertussis from clinical specimens
Regan-Lowe Medium
68
Used to select for Salmonella and some strains of Shigella from stool specimens.
Salmonella-Shigella Agar
69
Enriched medium used for the isolation of anaerobic bacteria
Schaedler Agar
70
Enrichment broth used for the recovery of low numbers of Salmonella and some strains of Shigella from stool and other specimens containing large amounts of mixed bacteria
Selenite Broth
71
Useful in the differentiation of Streptococci. It primarily distinguishes Enterococcus spp. (positive) from group D streptococci (negative)
Sodium Chloride Broth, 6.5%
72
primary isolation media for Mycoplasma spp. Biphasic media provide microaerophilic and moist conditions, which some Mycoplasma spp. prefer.
SP-4 Broth and SP-4 Agar
73
Selective medium used to isolate streptococci, primarily to detect β-hemolytic streptococci in throat swabs.
Streptococcus-Selective Agar
74
Enrichment medium used for recovery of Salmonella, except serotypes Typhi and Arizonae from stool specimens.
Tetrathionate Broth
75
All-purpose medium that can be used to isolate a wide range of bacteria
Thioglycollate Broth, Basal and Enriched
76
Selective medium used to isolate Vibrio spp. from stool specimens having mixed biota.
Thiosulfate Citrate Bile Salts Sucrose Agar
77
Selective differential medium useful in isolating and identifying C. diphtheriae from specimens containing mixed biota
Tinsdale Aga
78
Used to grow streptococci for serotyping. The selective formulation can be used to isolate group B streptococci from vaginal and rectal swabs
Todd-Hewitt Broth With Gentamicin and Nalidixic Acid
79
Used to determine whether a gramnegative rod is a glucose fermenter or non–glucose-fermenter, a fundamental characteristic in the initial classification of gram negative rods.
Triple Sugar Iron Agar
80
An all-purpose medium that supports the growth of many organisms. It is frequently used as the basal medium for sheep blood agar plates
Trypticase Soy Agar
81
All-purpose medium that supports the rapid growth of most organisms, including streptococci, without added supplements
Trypticase Soy Broth
82
Used for performing the indole test, a procedure that is particularly useful in identifying species of Enterobacteriaceae and identifying non–glucose-fermenting, gram-negative rods.
Tryptophan Broth, 1%
83
Detect an organism’s ability to hydrolyze urea. This is particularly useful in identifying species of Enterobacteriaceae
Urea Agar and Broth
84
Selective or nonselective (depending on the addition of antimicrobial agents) enrichment medium useful in the isolation of G. vaginalis.
Vaginalis Agar (V agar)
85
Selective, differential, primary plating medium used to isolate Salmonella and Shigella spp. from stool and other specimens containing mixed biota.
Xylose-Lysine-Desoxycholate Agar

CLINICAL BACTERIOLOGY (2 decks)

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