Culture Medium Flashcards
(57 cards)
1
Q
Differential medium for E. coli and Shigella
A
Acetate Agar
1
Q
- Acetate Agar (+) Growth
- Acetate Agar (-) Growth
A
- E. coli and enterobacteriaceae
- Proteus, providencia, shigella
2
Q
Enrichment medium for Vibrio and Aeromonas spp. from stool specimens
A
Alkaline peptone water
3
Q
Egg-based medium used for isolation of M. tuberculosis
A
American Trudeau Society Medium
4
Q
Components of American Trudeau Society Medium
- Provide fatty acid
- Provide carbon source
- Inhibitory for normal bacterial flora
A
- Egg
- Potato
- Malachite green
5
Q
- Selective differential for isolation and identification of members of the Bacteroides fragilis group
A
Bacteroides bile esculin agar
6
Q
- Bacteroides bile esculin agar (+) growth
- Bile esculin
A
- Dark brown or black colonies
- Darkening of the medium
7
Q
- Selective differential agar to isolate and identify group D streptococci and enterococci
A
Bile esculin agar
8
Q
Selective medium for the isolation of salmonella
A
Bismuth sulfite agar
9
Q
- Bismuth sulfite agar (black colonies with metallic sheen)
- Bismuth sulfite agar (Light green)
A
- Salmonella typhi
- S. gallinarum, choleraesuis & paratyphi
10
Q
- Enriched medium useful for the isolation of fastidious anaerobes
- Useful enrichment medium for the isolation of moderately fastidious, obligate anaerobes
- Selective enrichment medium rec. for the isolation of species of Bacteroides and Prevotella melaninogenica
- Primary isolation of obligate gram-negative anaerobes, particularly Bacteroides spp.
- Enrichment medium in recovery and the demonstration of β- hemolysis by Haemophilus spp. and Gardnerella vaginalis
- Routine medium to cultivate moderately
fastidious organisms
A
- Blood agar, anaerobic, CDC
- Blood agar, anaerobic, brucella base, wadsworth
- Blood agar, Anaerobic with Kanamycin and Vancomycin (KV)
- Blood agar, anaerobic, laked, with kanamycin, vancomycin and vitamin K (KVKL)
- Blood Agar, rabbit
- Blood agar, sheep
11
Q
Selective enrichment medium for isolation of Bacteroides, Prevotella, and other obligate anaerobes from specimens containing a mixture of obligate and facultative anaerobe
A
Blood Phenylethyl Alcohol Agar, Anaerobic, CDC (PEA)
12
Q
Selective enrichment medium for the isolation of B. pertussis and B. parapertussis
A
Bordet-Gengou Blood Agar
13
Q
- Enriched medium for the cultivation of non-fastidious and moderately fastidious microorganisms required for the cultivation of pneumococci for the bile solubility test
A
Brain Heart Infusion Broth
14
Q
Brain Heart Infusion Broth
Differentiates salt-tolerant enterococci from streptococci
A
6.5% NaCl
15
Q
- Enrichment medium useful in the isolation of Legionella spp., Francisella and Nocardia spp.
- Isolate **B. cepacia **selectively from RT specimens from patients with cystic fibrosis
- Selective enrichment medium for the isolation and cultivation of **Campylobacter spp. **from stool specimens
- Less inhibitory for Campylobacter spp.but more inhibitory for organisms found as normal fecal microbiota
A
- Buffered Charcoal Yeast Agar
- Burkholderia cepacia agar
- Campylobacter BA
- Campylobacter Charcoal Differential Agar
16
Q
- Flat, gray, nonhemolytic, raised and mucoid colonies
- Tan or slightly pink colonies swarming or spreading across the surface of the plate
A
- Campylobacter spp.
Campylobacter BA
17
Q
- Selective liquid medium used to enhance the isolation of Campylobacter spp.
- Select for the isolation of ** Yersinia enterocolitica** in stool samples
- Select for P. aeruginosa in specimens with mixed microbiota
A
- Campylobacter Thioglycollate Broth
- Cefsulodin-Irgasan Novobiocin
- Cetrimide agar
18
Q
Cefsulodin-Irgasan Novobiocin
(+)
A
Yersinia spp.
Aeromonas spp.
19
Q
Cetrimide agar
(+) under UVL: yellow-green fluorescence-because of pyoverdin production stimulated by low iron
A
P. aeruginosa
20
Q
Enrichment agar useful in promoting the growth of
Haemophilus and other fastidious bacterial species
A
Chocolate agar
21
Q
Chocolate agar
(+) growth
A
N. gonorrhoeae
Haemophilus spp.
22
Q
Selective, differential, chromogenic for isolation &
identification of yeast, S. aureus, and E. coli O157:H7
A
CHROME-agars
23
Q
Differentiating gram (-) enteric
bacilli
A
Citrate Agar, Simmons
24
# Citrate Agar, Simmons
(+) color growth result
Blue
25
Aerobic and anaerobic bacterial
organisms
Columbia Agar with and
without 5% Sheep Blood
26
Selective differential medium for the isolation and ID of gram (-) enteric bacteria
Eosin–Methylene Blue Agar (EMB)
27
# Eosin–Methylene Blue Agar (EMB)
* (+) blue-black colonies with a metallic greenish sheen
* (+) pink colonies
* E. coli
* Enterobacter sp.
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# Eosin–Methylene Blue Agar (EMB)
(-) nonfermenter colonies
| anu color
translucent and colorless or light purple
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Differential medium used to determine bacterium’s ability to produce gelatinase and thereby hydrolyze gelatin
Gelatin medium
30
# Gelatin medium
(+)
(-)
* Forms a gel
* Remains liquid
31
Selective enrichment medium used to enhance the chance of recovering **enteric pathogens, (Salmonella and Shigella)** from fecal specimens
Gram-Negative Broth
32
Selective differential medium for direct
isolation of enteric pathogens from feces
and for indirect isolation from selective
enrichment broth
Hektoen enteric agar (HEA)
33
# Hektoen enteric agar (HEA)
pH indicator
Bromothyl blue
34
# HEA
* (+)
* (-)
* Fermenters; one or both CHO E. coli (bright orange to salmon pink)
* Non-fermenters Salmonella & Shigella spp. (green to blue-green colonies)
35
Isolation of S. agalactiae, usually from vaginal or rectal swabs obtained from pregnant women
Lim broth
36
Recovery and identification of **Clostridium diphtheriae** promotes the development of characteristic metachromatic granules
Loeffler Coagulated Serum Slant
37
Cultivates Mycobacterium spp
Löwenstein- Jensen Medium (LJ)
38
(+): Colonies surrounded by small holes
containing a liquefied medium
Loeffler Coagulated
Serum Slant
39
Measures three parameters useful in
identifying species of
Enterobacteriaceae—(1) lysine
decarboxylation, (2) lysine deamination,
and (3) H2S production
Lysine Iron Agar
40
LIA pH indicator
Neutral red
41
# MacConkey agar (MAC)
* fermenters
* nonfermenters
(+) pink to red colonies
(-) colorless
42
Isolates E. coli O157:H7 (doesn’t
ferment sorbitol rapidly)
MacConkey Sorbitol Agar
43
ID of species of Enterobacteriaceae,
particularly Salmonella
Malonate Broth
44
# Malonate Broth
(+)
(-)
(+) prussian blue
(-) green
45
# Malonate Broth
pH indicator
Bromothyl blue
46
A selective and differential primary culture
medium useful in the recovery and ID of
staphylococci from specimens with mixed
microbiota
Mannitol Salt Agar
47
# Mannitol Salt Agar
* pH indicator
* (+)
* (-)
* Phenol red
* Yellow (staph)
* Red
48
Differentiating among
Enterobacteriaceae
Methyl Red Voges-
Proskauer Medium
49
* Cultivates Mycobacterium spp.
* Isoniazid-resistant strains grow better on these media
Middlebrook 7H10
and 7H11 Agars
50
More selective for mycobacteria because of added antimicrobial agents
Mitchison 7H11 Selective Agar
51
Selective enrichment medium used for
recovering Neisseria gonorrhoeae and
Neisseria meningitidis from specimens that
have mixed microbiota
Modified Thayer-Martin Agar
52
Testing the susceptibility of organisms to
antimicrobial agents testing starch hydrolysis
Mueller-Hinton Agar
53
Detect methicillin-resistant Staphylococcus aureus (MRSA)
Mueller-Hinton Agar
with 2% NaCl
54
Screen S. aureus isolates selectively for
resistance to oxacillin or nafcillin
Mueller-Hinton Agar
with 4% NaCl and 6
µg Oxacillin
55
Selective, differential, primary plating
medium used to isolate Salmonella and
Shigella spp. from stool and other
specimens containing mixed biota
Xylose-lysine-desoxycholate (XLD) agar
56
