Lipid Granules staining, Nuclear body staining, Polar stain

Question 1

Lipid Granules staining

• Components
• Fat globules
• Cells

Answer 1

• Sudan Black B stain, Xylol, 0.5% Safranin
• Blue-black/dark, bluish-gray/black
• Red

Question 2

Nuclear Body Staining

• Components

Answer 2

• 1% Mercuric chloride
• 1% crystal violet (pH 6-8)
• 2-5% Nigrosin (pH 3-4)

Question 3

Polar Stain

Answer 3

Wayson’s stain

Question 4

Wayson’s

• Solution A
• Soln B
• Polar Bodies

Answer 4

Soln A:
* Basic fuchsin - 0.2 g (90% dye)
* Methylene blue - .75g (90% dye)
* Ethyl alcohol - 20mL (95%)

Soln B:
* 5% phenol - 200 mL

Test result
* Purple