Culture System Flashcards
(33 cards)
Has exact known composition
Synthetic
Contains at least 1 non-chemically defined component
Non-synthetic / Complex
Used for organisms that cannot grow in cell free media
Tissue
From mouse cell line
Mccoy cell
From American green monkey
Vero cells
From lung carcinom
A549 cell
From cervical carcinoma
Hela cell
From laryngel carcinoma
Hep-2 cell
Supports the growth of non-fastidious organisms
Selective / General medium
Select growth of PARTICULAR organism and prevents other organisms through inhibitors
Selective medium
Selective medium used for Mycobecterium tuberculosis
Lowenstein jensen
Selective used for gram positive microorganisms
Phenylethyl alcohol
Selective media used for Neisseria gonorrhea
- Thayer Martin
- Martin Lewis
- New York City
Culture media that contains nutrient supplements
Enriched media
Can enhance the growth of microorganisms
Enrichment medium
Enrichment medium for Vibrio
Alkaline peptone water
Enrichment medium for Salmonella and Shigella
Selenite and Tetrathionate Broth
Human blood type used in blood agar plate
Type O
Medium that allows certain types of organisms to grow and differentiate
Selective-Differential medium
Selective-differential medium used to differentiate S. aureus from S. epidermidis/saprophyticus
Mannitol Salt Agar
Selective-differential medium for Vibrio spp.
Thiosulfate Citrate Bile Salt
Provides distinct colonial appearance for identification of microorganisms
Differential media
Three sugars in TSI (Triple Sugar Iron)
- Glucose
- Sucrose
- Lactose
Green zone around colony
Alpha hemolysis
