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Flashcards in Culturing Deck (33)
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1

3 types of culture media

1. Agar plates
2. Agar/slant tubes
3. Broth "liquid" tubes

2

General all purpose media that supports the growth of most bacteria
Used in high volume labs
Ex: Nutrient agar, Trypticase soy agar

Basic Nutrient Media

3

A basic nutrient media that is good for growth of less fastidious (picky) bacteria

Nutrient agar

4

A basic nutrient media that will support the growth of most clinical bacteria

Trypticase soy agar

5

Type of media that is basic nutrient media with extra nutrients that help grow fastidiouc (picky) bacteria

Enriched media

6

An enriched media that is very common and contains 5% sheep blood (blood cells in sheep blood are broken down quickly) in trypticase soy agar
Supports the growth of most pathogens

Blood agar plate (BAP)

7

An enriched media which blood is added to and then heated and used to grow organisms that cause STDs (Ex: Brucellosis)

Chocolate agar

8

Type of media that contains some type of substance that selects for one type of bacteria and inhibits other bacteria from growing

Selective media

9

A type of selective media that selects for Gram + bacteria

Phenylethel alcohol agar (PEA)

10

Very popular selective media that selects for GNROD only
Good to use with a lot of different bacteria

Macconkey agar (MAC)

11

Type of media that selects for a group of bacteria AND contains an indicator (visible change by the metabolic activity of the bacteria)

Differential media

12

Type of differential media that colonies change color if bacteria FERMENT LACTOSE
Pink = lactose fermentor
Clear = non-lactose fermentor

Macconkey (MAC)

13

Type of differential media that selects for Gram - enteric pathogens and inhibits all normal flora
Good for salmonella and shigella
Checks for sugar fermentors = salmon colored
Non sugar fermentors = blue/green

Hektoen-Enteric agar

14

A differential media that is used to classify bacteria according to their ability to ferment
Yellow = indicates fermentation
Red = no fermentation

Triple Sugar Iron (TSI)

15

A differential media that selects for genus Staphylococci and can be used to isolate Staph aureus
Yellow = organism can ferment mannitol
Red = non-pathogenic staph

Mannitol Salt Agar

16

Media type that is usually a broth and selects for growth of particular organisms and contain extra nutrients to allow bacteria to grow better
Used for fecal cultures

Selective broth media

17

Broth that selects for and enriches enteric pathogens and inhibit normal bowel flora

Selenite

18

Broth that suppresses coliform (gut) organisms

Tetrathionate broth

19

Misc media that is a useful, all purpose broth and used to increase the number of organisms before they are plated onto a solid medium

Brain-heart infusion

20

Misc broth media that determines motility of bacteria

Motility media

21

Misc media that tests for detection of a bacteria's ability to produce indole

Indole test media

22

Creates partial hemolysis of blood agar
Narrow band of green, slimey discoloration around bacterial growth

Alpha

23

Creates complete hemolysis of blood agar
Clear band around bacterial growth

Beta

24

Created by non-hemolytic bacteria
No change of blood agar

Gamma

25

Double zoned hemolysis
Not common

Delta

26

Which quadrant of streaking is where most isolated colonies grow?

D

27

What temp and how long should plates be incubated?

35-37* C, 24-48hr

28

Sensitivity test that utilizes Mueller-Hinton media
Tests resistance to Abx

Kirby Bauer

29

The larger the zone of inhibition, the more ____________ the bacteria is to the abx

susceptible

30

R

Resistant