Cytology / Hystology of the eye Flashcards
Main aspects that we should be aware of (19 cards)
Squamous cell carcinoma or Corneal epithelial hyperplasia?
Clinical atlas of small animal cytology and hematology pg 406
Corneal epithelial hyperplasia
Higher N/C ratios and more basophilic (haematoxylin) cytoplasm
What is the likely diagnosis?
Clinical atlas of small cytology… pg 407
Corneal Squamous cell carcinoma
Variable volume and bright sky-blue cytoplasm.
Histopathology is often required for definitive diagnosis
a) What is the likely diagnosis?
b) What species often present this ocular disorder?
c) Grossly description?
Clinical atlas of small cytology… pg 408
a) Eosinophilic Keratitis
b) Cats
c) pink/white plaques with gritty, yellow material, most commonly affects the superotemporal quadrant
Others: small mature lymphocytes, mast cells, non-degerative neutrophils
a) What is the likely diagnosis?
b) ethiologic agent
Clinical atlas of small cytology… pg 410
Chlamidial conjunctivitis
basophilic perinuclear inclusions associated with epithelial cells
a) What is the likely diagnosis?
b) ethiologic agent
Clinical atlas of small cytology… pg 411
a) Mycoplasmal conjunctivitis
b) Mycoplasma felis
basophilic organisms scattered (surface squamous epithelial cell)
a) What is the likely diagnosis?
b) DDx?
c) Cell type common in dogs and cats?
d) Grossly description?
e) Prognosis
a) conjunctival lymphoma
b) neoplasms affecting conjunctiva: SCC, melanoma, mast cell tumor, papilloma
c) T-cell = dogs ; B-cell = cats
d) Diffuse thickening of the conjunctiva, discrete masses
e) better for conjunctival than intraocular
Large nuclei and prominent nucleoli, clear vacuoles in the cytoplasm
a) What’s your diagnosis?
b) Identify circled structure
c) Grossly description?
COPLOW
a) Persistent fetal vasculature: persistent pupillary membranes
b) iris-like tissue are extending from the iris and cross the pupil: pupillary membranes
c) Thin, brown membranes branch from the iris and arborize across the pupil
What’s your diagnosis?
COPLOW
Optic Nerve Coloboma
a) What’s your diagnostic?
b) Identify the structures (arrow and circle) on the top left image
COPLOW
a) Hypervicosity syndrome
b) arrow = Detached retina and intraocular hemorrhage; circle = Opaque subretinal fluid
a) What’s your diagnostic?
b) Ethiologic agent
COPLOW
a) Encephalitozoonosis
b) Encephalitozoon cuniculi
a) What’s your diagnosis?
b) What’s the circled structure?
COPLOW
a) Cryptococcosis
b) Yeast
Yeast = levedura
Identify the structures:
GELATT: Ophthalmic anatomy p.48
Globet cells
palpebral conjunctiva of a porcine eyelid: stratified to pseudostratified columnar epithelium
Classify the corneal epithelium considering its hystologic features:
GELATT: ophthalmic anatomy p. 54
Nonkeratinized, stratified squamous epithelium
Identify each structure:
GELATT: ophthalmic anatomy p. 56
Superficial squamous cells
Wing cells
Basal cells
Basal lamina + corneal nerve
Basement membrane
Anterior stroma
The basal cells are firmly attached to the basal lamina of the basement membrane by…
GELATT: ophthalmic anatomy p. 56
Hemidesmosomes,
anchoring collagen fibrils,
glycoprotein laminin
a) Identify the tissue
b) Identify the structure
c) In what species it can be found?
GELATT: ophthalmic anatomy p. 59
a)Cornea
b)Bowman’s layer
c)Birds, giraffes, dolphins, some whales, large herbivores
a) Rhesus monkey; b) Dolphin; c) whale; d) giraffe
Considering the images below, identify the ethiologic agents
Left side: Chlamydia
Right side: mycoplasm
iDENTIFY THE TISSUE AND THE STRUCTURES POINTED BY THE ARROWS
Conjunctival lymphoid follicle
Histoplasma spp
Identify the ethiologic agent
Young_OcCyt
** Leishmania spp**
