Day 8

Question 1

Slow growers ; require several weeks to see visible colonies; high concentrations of loped in cell wall

Answer 1

Acid fast Bacilli

Question 2

What does acid fast bacilli require to grow?

Answer 2

Increased CO2 and 37degrees Celsius

Question 3

What is the microscopic appearance of acid fast bacilli?

Answer 3

Slightly curved or straight bacilli; may see mycelia

Question 4

What are the 4 classifications for AFB?

Answer 4

Runyon Group 1 Photochromogen
Runyon Group 2 Scotochromogen
Runyon Group 3
Runyon Group 4

Question 5

which Runyon Group is described?

Produce pigment after exposure to light

Answer 5

Group 1

Question 6

which Runyon Group is described?

Produce pigment in the dark and light.

Answer 6

Group 2

Question 7

which Runyon Group is described?

Slow growing; nonpigmented

Answer 7

Group 3

Question 8

which Runyon Group is described?

Rapid growers

Answer 8

Group 4

Question 9

What is the etiological agent of tuberculosis?

Answer 9

Mycobacterium tuberculosis

Question 10

Causes human and animal tuberculosis; acquired through unpasteurized milk and undercooked beef

Answer 10

Mycobacterium bovis

Question 11

Causative agent of Hansen’s disease(leprosy) ; causes skin lesions

Answer 11

Mycobacterium leprae

Question 12

Most common mycobacterium found in AIDS patients in US

Answer 12

Mycobacterium avium-intracellulare

Question 13

What is the most widely used specimen for mycobacterium?

Answer 13

Sputum

Question 14

For decontamination and digestion, what are the 3 chemicals used?

Answer 14

Sodium hydroxide (2-4%)(mostcommon)
Zephaniah-trisodium-phosphate(13%)
Permits better recovery of organisms
N-acetyl-L-cysteine
Best for digestion of mucoprotein

Question 15

What media is used for AFB?

Answer 15

Egg and potato based

Question 16

Contains machalite green inhibitory dye

Answer 16

Lowenstein Jensen

Question 17

Contains a higher concentration of machalite green that LJ

Answer 17

Pertragnani medium

Question 18

Detects early growth of microscopic colonies; used for antimicrobial sensitivity testing

Answer 18

Middlebrook 7H10

Question 19

Name a partially acid fast bacilli

Answer 19

Nocardia spp.

Question 20

Most common sexually transmitted bacterial agent; associated with:
Trachoma
Trachoma Inclusion Conjunctivitis(TIC)
Lymphogranuloma venereum(LGV)
Genital tract disease

Answer 20

Chlamydia spp.

Question 21

Chlamydia species that mainly affects birds

Answer 21

Chlamydia psittaci

Question 22

Chlamydia species that is isolated in patients with respiratory infections

Answer 22

Chlamydia pneumoniae

Question 23

How do you collect Chlamydia specimens?

Answer 23

By vigorously scraping the involved epithelial cells; cervical and urethral scrapings

Question 24

What spp. requires embryonated chicken egg cell cultures

Answer 24

Chlamydia

Question 25

What is the most common method of detection of Chlamydia?

Answer 25

ELISA and immunofloresence

Question 26

What class does Mycoplasma belong too?

Answer 26

Mollicutes

Question 27

“Fried egg” appearance; Supravital dyes (Dienes method) dark blue center and light blue edges

Answer 27

Mycoplasma spp.

Question 28

What organism is asymptomatic And known as “walking pneumonia “?

Answer 28

Mycoplasma pneumoniae

Question 29

Causes spontaneous abortions and post-partum fever and respiratory diseases in neonates

Answer 29

Mycoplasma hominis

Question 30

What does mycoplasma spp. Need to grow

Answer 30

95% nitrogen and 5%CO2