denaturation of proteins Flashcards
(7 cards)
1
Q
What is denaturation?
A
The alteration of tertiary structure of a protein; this loss of the three-dimensional shape of the protein is often irreversible and the protein is no longer functional.
2
Q
What is denaturation usually as a result of?
A
Breaking down of hydrogen and ionic bonds.
3
Q
What is denaturation caused by?
A
High temperature above the optimum, extreme changes in pH, and heavy metals.
4
Q
Which bonds are not often broken in denaturation?
A
Disulfide.
5
Q
What is the test for proteins?
A
Biuret test.
6
Q
What colour indicates proteins are present?
A
Purple or lilac.
7
Q
What colour indicates proteins are not present?
A
Blue.
