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Dengue Flashcards

1
Q

What do you detect in serologic tests for dengue

A

IgG ,IgM, NS1 antigen (non-structural protein 1)

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2
Q

Severe form of Dengue Fever

A

Dengue Hemorrhagic Fever

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3
Q

Single positive stranded RNA virus

A

Dengue Virus

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4
Q

What mosquito transmits dengue?

A 
Aedes aegypti (mainly)
Aedes albopictus (ocassionally)
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5
Q

Where is A. aegypti usually located?

A

urban, suburban. manmade containers like tires, flower pots, household water

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6
Q

4 serotypes

A

DENV 1,DENV-2, DENV-3,DENV4

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7
Q

recovery from infection is believed to provide LIFELONG IMMUNITY against serotype

A

TRUE

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8
Q

CROSS-IMMUNITY to other serotypes after recovery is only partial and temporary

A

TRUE

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9
Q

transported to america & eurpose by asian products like bamboos & trees

A

A. albopictus

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10
Q

Secreted into blood during dengue infection

A

NS1

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11
Q

When is NS1 detectable ?

A
  • Acutephase *
    within 1-2 days ff infection ;9 days ff symptom onset
    *SECONDARY dengue virus infection
    1-4 days ff symptom onset
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12
Q

High titers as seen in primary infection

A

IgM

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13
Q

Classified as presumptive recent dengue virus infections

A

Positive IgM result

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14
Q

Detectable 4- 5 days after onset symptoms and detectable approx. for 12 wks

A

IgM

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15
Q

by 3 weeks ff.exposure of this type of antibody, nearly all immunocompetent individuals should have develop IgG antibodies to DENV

A

igG

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16
Q

presence of this type of antibody indicates exposure to the virus sometime in the past

A

igG

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17
Q

Principle of rapid test kits for Dengue

A

Lateral flow immunochromatographic assay

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18
Q

Dengue Shock syndrom treatment

A
  • hospitalization in an intensive care unit for close monitoring and administration of medication that raises Blood pressure into normal range
  • IV fluids
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19
Q

Increased permeability of blood vessels

A

plasma leakage

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20
Q

DHF 4 key features

A
  • Increased permeability of Blood vessels
  • Low blood platelet count
  • fever lasting 2-7 days
  • tendency toward severe hemorrhagic bledding
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21
Q

Most dangerous symptom of Dengue

A

Increased permeability of blood vessels leading to plasma leakage that may cause SHOCK

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22
Q

inability to circulate blood properly bcs there is not enough fluid left in the blood vessel is

A

SHOCK

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23
Q

shock can lead to

A
  • organ failure

* metabolic problems

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24
Q

Where and when was the first vaccine of Dengue made available

A

Mexico, December 2015

25
Age bracket for Dengue vaccine
9-25 yrs old( 3 dose series )
26
Diagnosis of early acute dengue infection
Dengue NS1 Ag test device
27
designed for the rapid qualitative, differential detection of antibodies to dengue virus
IgG/IgM test device
28
Conjugate pad for Dengue Ns1 Ag test device
mouse monoclonal anti-dengue NS1-gold colloid
29
NS1 -Test line
mouse monoclonal anti-dengue NS1
30
Control line for NS1 test device
goat anti-mouse IgG
31
Conjugate pad for Dengue IgG/IgM test device
recombinant dengue virus envelope protein-gold colloid
32
Test line "G" for Dengue IgG/IgM test device
mouse monoclonal antihuman IgG
33
Test line "M" for Dengue IgG/IgM test device
mouse monoclonal antihuman IgM
34
Control line for Dengue IgG/IgM test device
rabbit anti-dengue IgG
35
Absence of Control Bands
Invalid
36
Dengue NS1 antigen positive test result
purple colored T band
37
When specimen is added into sample well, what mechanism of action happens when spx migrates across the cassette
capillary action
38
Specimen for dengue test
Fresh non hemolyzed serum obtained by venipuncture
39
Interpret results of Dengue NS1 Antigen and IgG/IgM
15-20 minutes
40
C- Band is present and absence of color in Dengue NS1 antigen
Negative result
41
Presence of C- band and T-band in Dengue NS1 Test kit
POsitive result
42
No C-band is developed but shows purple color in T-band
Invalid
43
Repeat assay using a new device
invalid result
44
Dengue IgG / IgM negative result
Retest in 3-5 days if dengue is suspected
45
only C -band is present in Dengue IgG/IgM test kit
Negative
46
Presence of C-band, "M" band is developed in Dengue IgG/IgM test kit
IgM Positive (Primary dengue Infection)
47
Presence of C -band, "G" band is developed in Dengue IgG/IgM test kit
IgG positive ( Secondary or Past Dengue infection)
48
Result of M / G line is weak in Dengue IgG/IgM test kit
Positive result
49
Presence of C-band , "G" and "M" bands in Dengue IgG/IgM test kit
Both IgG and IgM positive (Late primary or early secondary dengue infection)
50
Most likely reasons for control line failure
- Insufficient spx volume | - Incorrect procedural technique
51
a negative test result cannon exclude a recent infection
TRUE
52
presence of detectable NS-1 antigen
may mean positive for early dengue infection
53
detectable levels of IgM may be LOW
early primary infections and some secondary infections
54
Some patients may nit produce detectable levels of antibody within how many days
first 7-10 days after infection, when symptoms persist px should be retested 3-4 days after the first specimen
55
Expected to be detected 1 day after onset of fever and persist up to 9 days in both primary and secondary infection
NS-1 antigen
56
anti-NS 1 antibodies are produced
detection of NS-1 antigen is inhibited
57
characterized by the presence of detectable IgM 3-5 days after onset of symtomps
Primary dengue
58
characterized by presence of specific IgG 1-2 days after onset of infection, generally accompanied by elevation of IgM
Secondary dengue
59
Serological cross reactivity across the Flavivirus is common
DI- SAD-JOD-WEgtengen-YEs! - Dengue virus - St.Louis encephalitis - Japanese encephalitis, - West nile & yellow fever virus

Immuno-sero (4 decks)

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