Department Images 152 - 209 (Dustin) Flashcards by Dustin O

What organ is this from?

What does the arrow point to?

Star?

1, 2, 3?

Ovary

Arrow: Peritoneal Epithelium (simple cuboidal)

Star: Graafian Follicle (although it’s labeled as a Tertiary Follicle, but looks much like a deeper Graafian. Gonna ask about this one)

1: Primary Follicles
2: Primordial Follicles
3: Secondary Follicle (probably)

(lemme know if you think one of these is wrong! if I remember correctly, the Hungarian way to classify these is slightly different than in English books, maybe more similar to German books)

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Where is this?

What type of epithelium do you see?

What’s the layer beneath it?

What are the round things?

Ovary

Peritoneal Epithelium (simple cuboidal)

Layer beneath it = Tunica Albuginea

Round things = Primordial Follicles

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What type of follicles are the 3 large ones in the bottom half of the image?

And the medium-sized one right in the middle?

And all the little ones at the top right?

3 large ones: Secondary Follicles

Medium sized in middle: Primary Follicles

Top ones: Primordial Follicles

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What kind of follicle?

Graafian

But the image is labeled as Tertiary Follicle, going to ask about this later

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What is this from?

What are the paler cells that make up most of the image?

What are the darker cells at the top?

Corpus Luteum

Paler cells = Granulosa Lutein Cells

Darker cells = Theca Lutein Cells

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What tissue is this from?

Uterine Tube / Oviduct, Ampullary Part

Simple Columnar, some ciliated and some non-ciliated

In some slides, the mesosalpynx might be seen: double layer of peritoneum containing blood vessels

Don’t mix it up with seminal vesicle

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What tissue is this?

What type of cells do you see in the epithelium?

Oviduct, Ampulla

See Simple Columnar Epithelium with:

Ciliated Cells

Non-ciliated (secretory) Cells

“Peg” or “Pin” Cells - dark, elongated nuclei protruding into the lumen, degenerating cells

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What tissue is this from?

Uterus - Proliferative Phase (early in phase)

Has relatively less glands than secretory phase, thinner endometrium

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What tissue is this?

Uterus - Secretory Phase

Has many “tortuous” glands, thick endometrium

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What tissue is this from?

What layers do you see?

Fetal portion of the Placenta

At the lowest part of the image is the simple cuboidal amnion epithelium

Above that is the chorionic plate: thick connective tissue

At the top you see chorionic villi

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What tissue is this from?

1? 2?

What’s the space between the structures?

Placenta

1: Chorionic Villi (free)
2: Anchoring Villus

Intervillous Space - fills with maternal blood

Note also the syncitiotrophoblasts, cytotrophoblasts and other important placental structures

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What tissue is this?

What does the image show?

Placenta

Shows the anchoring villus connecting with the basal plate and decidual cells, with eosinophilic fibrinoid

(I think, anyway)

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What’s the big pink blob?

Placental Septum with mainly decidual cells

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What tissue is this?

What does this show?

Placenta

Shows the anchoring villus connecting with the basal plate and decidual cells, with eosinophilic fibrinoid

(another one)

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What tissue is this?

Vagina

Note:

Wide lamina propria mucosae has lots of CT with elastic fibers
Vaginal wall has neither glands nor submucosa
Esophagus easily distinguished from vagina bc esophagus has muscularis mucosae, tela submucosa, and glands
Ureter has transitional epithelial lining and regular smooth muscle in its tunica muscularis

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What tissue is this from?

Non-Lactating Breast

(Mamma Nonlactans)

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What tissue is this?

Lactating Breast

Mamma Lactans

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What tissue is this?

What glands are here?

Non-Lactating Breast

There are Areolar Glands of Montgomery here: apocrine secretion

Areolar glands or Glands of Montgomery are sebaceous glands in the areola surrounding the nipple. The glands make oily secretions (lipoid fluid) to keep the areola and the nipple lubricated and protected. Volatile compounds in these secretions may also serve as an olfactory stimulus for newborn appetite.

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What tissue is this from?

What stain?

Be able to name the parts

Hypophysis

Chrome-Hematoxylin-Phloxin stain: helps differentiate between acidophils and basophils of the adenohypophysis (the bright red tissue to the right)

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What tissue is this from?

What cells do you see?

Adenohypophysis - H-E Stain

Acidophils: bright red

Basophils: larger, basophilic cells

Chromophobes: poorly stained

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What tissue is this?

What cells do you see?

Adenohypophysis

(with Chrome-Hematoxylin-Phloxin stain to show more of a difference between cells)

Acidophils: bright red

Basophils: larger blue cells

Chromophobes: poorly stained

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What tissue is this?

Adenohypophysis

(Says “Kresazan” for stain, which either google translates as “road traffic,” or it’s some sort of altered Azan stain)

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What tissue is this?

Adenohypophysis

(Says “Kresazan” for stain, which either google translates as “road traffic,” or it’s some sort of altered Azan stain)

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What 2 parts of the pituitary gland do you see here?

Study These Flashcards

Intermediate Part to the left

Neurohypophysis takes up the rest of the image

What tissue is this? What important cell types/structures are here?

**Neurohypophysis** Look for **pituicytes**: special glial cells, only their oval pale nucleus can be seen I don't think the **Herring Bodies** are supposed to be easily visible on the regular H-E stain, but with the chrome-hematoxylin-phloxin stain they should be dark greyish-blue, irregular spots

What tissue is this from? What are the characteristic features you see of that tissue?

**Pineal Gland** There are a few places of **brain sand / acervulus** Also see **pinealocytes** with large, oval, granular nuclei and prominent nucleoli Plus **interstitial glial cells** between pineaolocytes and blood vessels

What tissue is this? What parts and cell types do you see?

**Thyroid Gland** Look at answer image for identification

What tissue is this?

**Thyroid Gland** again This time the image says "Autoradiographia"

What tissue is this? What 2 cell types should you be able to identify?

**Parathyroid Gland** Identify **oxyphils** and **chief cells**

What tissue is this? What layer of that tissue makes up the majority of the image? What is the thinner layer towards the bottom of the image? And the lower-most layer?

**Adrenal Cortex** ## Footnote (image is upside-down from the normal order) Most of the image is **Zona Fasciculata** Below that is **Zona Glomerulosa** Then there's the **Capsule**

What organ is this from? What layer makes up the lower half of the image? What layer is the top-right corner?

**Adrenal Gland Cortex** Lower half of image is **Zona Fasciculata** Top-right = **Zona Reticularis**

What tissue is this from? What are the important layers/cells?

**Adrenal Gland** Shows **Zona Reticularis** and **Medulla** Notice the **ganglion cells** and **chromaffin** **cells** in the medulla

What tissue is this from? What formation do you see?

**Adrenal Gland Medulla** See the **sinusoid capillary**, feel the **sinusoid capillary**, know the **sinusoid capillary** probably some other important stuff too

What tissue is this? What stain? What are the different layers of lining called?

**Peripheral Nerve** **Osmium Tetroxid** stain Outermost lining = **epineurium**, then there's **perineurium** over each of the two balls, and **endoneurium** over individual nerve fibers

What is this tissue?

**Peripheral Nerve**

What's this round blob here?

**Peripheral Nerve**

Can you spot the peripheral nerve in this one?

Go on, find it! This is a scalp skin slide

What tissue is this?

**Spinal Ganglion** with **Pseudounipolar Neurons** ## Footnote Very large round cell bodies with nuclei that are poorly stained except for their nucleoli Perikarya should be basophilic due to **Nissl substance** Note also the: **Satellite cells** around the neurons **Schwann cells** whose nuclei are among the nerve fibers

Wtf is this thing

Another example of **pseudounipolar neurons** but with silver staining

What tissue is this? What stain?

**Autonomic Ganglion** with **Multipolar Cells** **Silver Nitrate Impregnation** The nuclei are "negatively stained"

What is the paler mass here?

This is a **pancreas** slide This is labeled as a **Vegetative / autonomic ganglion** and does not really look like the alpha/beta cells of islets of Langerhans

What slide is this? What stain?

**Spinal Cord Cross-Section** ## Footnote I think this only shows half of the spinal cord, with the dorsal horn being in the lower half of the image (flipped from how you probably normally would draw it) **Cajal Silver** stain

What are the most prominent type of cells you see here? What slide might they be in (tissue and stain)

**(Multipolar) Motor Neurons** Taken from the **ventral horn of the spinal cord** **Nissl stain** (with cresyl-violet), which gives the rER a bluish-purple (**Nissl Substance**)

What tissue is this?

**Cerebral Cortex** Remember the 6 layers (could be worse, i mean it's less layers than are in a burrito from taco bell) Note that the image just has one pyramidal layer and also a ganglionic layer, but our department usually calls these the **outer** and **inner pyramidal layers**

What tissue is this image from? What stain?

**Cerebral Cortex** **Bielschowsky** Stain Those 6 layers are more visible with this stain Note that the image just has one pyramidal layer and also a ganglionic layer, but our department usually calls these the **outer** and **inner pyramidal layers**

What tissue is this from? What stain? What notable cell is here?

**Cerebral Cortex - Golgi** stain **Pyramidal Cell** (maybe even a **Betz** cell, but hard to tell when it's zoomed in)

What tissue is this from? What is the light layer at the bottom and the darker layer above that? What are the big dark cells at the border of those 2 layers?

**Cerebellum** Light layer = **Molecular Layer** Dark layer = **Granule Cell Layer** Big cell in the border = **Purkinje Cell**, which make up a **Purkinje Cell Layer** aka **Stratum Ganglionare**

What tissue is this from? Stain? What is the layer at the bottom and the darker layer above that? What are the big dark cells at the border of those 2 layers?

**Cerebellum - Bielschowsky stain** Light layer = **Molecular Layer** Dark layer = **Granule Cell Layer** Big cell in the border = **Purkinje Cell**, which make up a **Purkinje Cell Layer** aka **Stratum Ganglionare**

What type of cell is this? What stain?

**Purkinje Cell** (in cerebellar cortex) **Golgi stain**

There are 2 types of astrocytes here to find

It's the **fibrous** and **protoplasmic astrocytes** This is a Cajal gold impregnation stain

What is the green microsoft paint arrow pointing at?

a **Meissner Corpuscle**

This thing is fucking crazy, what is it?

A **Pancreatic Paccinian Corpuscle** So *your pancreas can feel vibration*

What tissue is this? What stain?

**Motor End Plate** **Acetylcholinesterase Histochemistry stain** You can lightly see the muscle fibers, while the dark splotches are the motor end plates

What tissue is this from? There's a ton of shit here, be able to find it

This is the **Eye** with the **sclera, iris, etc** Make sure you know the **Canal of Schlemm**

What's this tissue from? What are all these layers?

**Retina**

What tissue is this from? What stain?

**Cochlea** **Semithin Section, Toluidine Blue** stain

What tissue is this from? Can you identify some of the cells?

**Organ of Corti** This image has all the important shit, but our school still tries to teach us all the names of the douchebags who wanted some phalangeal cells in the ear named after them like **Hensen, Deiter, Claudius,** and **Boettcher** Corti was the only one of those dudes who mattered

What tissue is this from?

**Lacrimal Gland** May be easy since this deck is separate from most of the other ones with gland cards, but it's similar to *parotid gland* and *pancreas* Although it's exclusively serous, it has much less adipose than the parotid gland, and of course it doesn't have islets of Langerhans

Department Images 152 - 209 (Dustin) Flashcards

(58 cards)