Department Images 98 - 151 (Dustin)

Question 1

What tissue is this slide from?

(really difficult without seeing more of the lumen)

Answer 1

Esophagus

contains inner circular and outer tongitudinal muscles layers with skeletal muscle interspersed throughout

epithelium is stratified squamous non-keratinized

Question 2

What tissue is this slide from?

Answer 2

Stomach Cardia

Near the esophageal-gastric junction, the bottom part of the image has strat squam non-keratinizing epithelium

Question 3

Which tissue is this slide taken from?

Answer 3

Fundus of Stomach

Has many different kinds of cells in the gastric pits: know mucus neck cells, parietal cells, chief cells

One protruding fold of cells is called a plica villosa, surrounded on either side by gastric pits

Question 4

What tissue is this from?

What stain? What is the purpose of this stain?

Answer 4

Fundus of Stomach

PAS-Congo-Hematoxylin stain

Makes mucus (both surface and neck) cells purple as they are PAS positive

Parietal cells turn orange

Chief cells become basophilic / grey

Question 5

What cells here are orange?

Which ones are red?

Answer 5

Orange = Parietal Cells

Red = Mucus Neck Cells

Question 6

What are the orange cells?

What are the greyer/ basophilic cells more at the top of the image?

What are the redder cells at the bottom of the image?

Answer 6

Orange = Parietal Cells

Greyer/basophilic = Chief Cells

Redder = Mucus Neck Cells

All in the gastric glands of stomach fundus

Question 7

What tissue is this slide from?

Answer 7

Pylorus of Stomach

has branched tubular glands, almost entirely mucus secreting cells, stained poorly

Question 8

What tissue is this slide from?

What major structures do you see?

Answer 8

Pylorus of Stomach

See Gastric Pits, and below are Pyloric Glands - not to be confused with Brunner’s glands of the duodenum which are in the tela submucosa

Question 9

What is the large fold you see curving rightward in the lower half of the image?

What tissue is this slide from?

Answer 9

Plica Circularis aka Fold of Kerckring

Slide is from Jejunum

(no Peyer’s Patches or Brunner’s Glands)

Question 10

What do you call the protruding fold of cells that makes up most of the image?

Answer 10

Intestinal Villi

Question 11

What tissue is this slide from?

What stain?

(not a typical slide)

Answer 11

Jejunum

Indian Ink

(book says it demonstrates the blood vessels of the villus)

Question 12

What are the cells with reddish granules at the base of the crypt?

Where do these exist in the GI tract?

Answer 12

Paneth Cells

Exist only in small intestine crypts of Lieberkuhn

Question 13

What do you call the lighter thing sandwiched between the two darker eosinophilic structures?

Answer 13

Myenteric Plexus (of Auerbach)

Takes place between the inner circular and outer longitudinal smooth muscle layers of the GI tract

Question 14

What tissue is this slide from?

Answer 14

Duodenum

Have typical small intestine structures + Brunner’s Glands in the tela submucosa

Question 15

What type of glands are at the bottom of the image?

What tissue is this slide from?

Answer 15

Brunner’s Submucosal Glands

From the Duodenum

Question 16

What tissue is this slide from?

What are the large purple areas in the middle?

Answer 16

Ileum

Purple areas = Peyer’s Patches

Question 17

What tissue is this slide from?

Answer 17

Colon

Crypts of Lieberkuhn with a bunch of goblet cells and no villi

Question 18

What tissue is this slide from?

Answer 18

Appendix

Has secondary follicles, NOT Peyer’s Patches

Many goblet cells, few/short crypts, no teniae

Question 19

What tissue is this image from?

What stain?

What is the majority of the blue stuff in the image?

Answer 19

Pig Liver (Azan)

Blue = interlobular connective tissue that clearly separates the hepatic lobules, and in some corners between lobules you see the (Glisson’s) portal triad of interlobular bile duct, vein, and artery

Question 20

What 3 important vessels do you see here?

What is the combination of them called?

What stain?

Answer 20

Interlobular Bile Duct: Simple cuboidal-columnar epithelium

Interlobular Artery/arteriole: thick-walled, small diameter.

Interlobular Vein/venule: largest diameter vessel here, typical thin wall

All 3 together = portal triad

Azan Stain

Question 21

What organ is this from?

What stain?

What does this stain show?

Answer 21

Liver, India Ink Injection

Shows vascular pattern / Kupffer cells

Question 22

What liver structure is this image focused on?

Answer 22

Portal Triad again

Really only the interlobular veins/venules are clear

Question 23

What 3 important vessels do you see here?

Answer 23

Portal Triad again

This time all 3 are very clearly here

Question 24

What type of cells are here?

Answer 24

Hepatocytes

Make up liver sinusoids, lined with Kupffer Cells which are generally not visible with an H-E stain like this

Question 25

What tissue is this? What stain? What does that stain show?

Answer 25

**Liver** **Best's Carmine Staining** Stains **glycogen** granules as red You know liver got that glycogen

Question 26

What organ is this? What stain? What animal is it from? What does the stain show?

Answer 26

**Liver** **Golgi silver impregnation stain** **Pigeon** (that was stressed for some reason) Stain shows the **bile capillaries between hepatocytes**. Note that there are **Hering ducts** at the periphery of hepatic lobules, but I don't think you can see them

Question 27

What organ is this from? What kind of epithelium? What is unique about its layers?

Answer 27

**Gall Bladder** **Simple Columnar** with **Cuticle** Has *no lamina muscularis mucosa or submucosa*

Question 28

What organ is this from? What are most of the cells you see? What is the lighter cluster at the top-right of the image?

Answer 28

**Pancreas** Most cells are **serous acini** (exocrine) Lighter area = **Islets of Langerhans** (endocrine function) Note there are also NO salivary ducts or adipose tissue, how you can differentiate from Lacrimal and Parotid Glands

Question 29

What type of cells are these? What organ are they from?

Answer 29

**Serous acini** of **Pancreas** Pretty bitchy question without more context, but pancreatic serous acini are supposed to be *more acidophilic and have a narrower lumen*

Question 30

What organ is this from? Be able to identify many regions, I'm not gonna label it here Note also the epithelium transitioning

Answer 30

**Larynx - Frontal Section** Epithelium everywhere *except the vocal folds* should be Respiratory (**pseudostratified columnar ciliated**) In vocal folds it is **stratified squamous non-keratinizing**

Question 31

Where in the body is this slide from? What type of epithelium? What type of tissue makes up the left half of the image?

Answer 31

**Trachea** **Pseudostratified Columnar Ciliated** (Respiratory Epithelium) Left half = **Hyaline (Tracheal) cartilage**

Question 32

What organ is this image from? 1, 2, and 3? What is the mostly white space between 3 and 3?

Answer 32

**Lung** 1: **Bronchus** because it has respiratory epithelium and *cartilage* 2: **Bronchiolus** because of smooth muscle 3: A **pulmonary artery** Between 2 and 3: **Alveoli**

Question 33

What type of airway is the most prominent in this image? (the big one with the irregular dark lumen)

Answer 33

**Terminal Bronchioles** Epithelium becomes simple *cuboidal* (mostly Club/Clara cells) non-ciliated with no goblet cells Should probably say Club cells and not Clara cells because Clara was a nazi, and you don't like nazis, do you?

Question 34

What type of airway is this?

Answer 34

Alveolar Sac with Alveoli Look for the Type I and Type II Pneumocytes

Question 35

What tissue is this? What stain? What does it show?

Answer 35

**Lung** **India Ink** - shows **pulmonary capillary network**

Question 36

What organ is this? What stain? What does it show?

Answer 36

**Lung** **Resorcin Fuchsin** - shows **elastic fibers** of the pulmonary connective tissue

Question 37

What organ is this from? And what part of that organ? What are the round things you see? What are the 2 layers of those things?

Answer 37

**Kidney - Cortex** Round stuff = **Renal / Malpighian Corpuscles**, which contain Bowman's capsule and glomerulus **Bowman's Capsule** has 2 layers: _Parietal_ (simp squam epith) _Visceral_ (**podocytes**)

Question 38

What organ is this from? What stain?

Answer 38

**Kidney** **India Ink**: shows the vascular pattern, staining the **interlobular arteries** and **glomerulus**

Question 39

What organ is this? There are some labeled things on the answer card, be able to know where all of them are on this image

Answer 39

**Kidney** (Cortex) Note: **macula densa** is a modified part of **distal convoluted tubule (DCT)** situated at the **vascular pole** of the renal corpuscle. Has darker cells, more nuclei The **DCT** have simple cuboidal epithelium with a wider, regular, rounder lumen than the PCT, and DCT lumen has no brush border

Question 40

From the top, what is the first labeled duct? Second? Third? Where is this sample from?

Answer 40

1st = **Thick Limb of Henle Loop** 2nd = **Thin Limb of Henle Loop** 3rd = **Collecting Duct** (I think, anyway..) This is from **outer medulla of kidney**

Question 41

What organ is this from? What type of epithelium? Be able to name layers

Answer 41

**Urinary Bladder** **Transitional Epithelium** (Esophagus can look similar, the umbrella cells are clear in this one though)

Question 42

What organ? 1 - 5?

Answer 42

**Testis** 1. **Convoluted Seminiferous Tubule** (make sure you say convoluted!) 2. **Leydig Cells** - large polygonal cells with acidophilic cytoplasm, pale nucleus, visible nuceolus 3. **Sertoli Cell -** pale conical nucleus, prominent nucleolus 4. **Spermatogonium** (closer to the wall of the tubule) and **Primary Spermatocyte** 5. **Secondary Spermatocyte**

Question 43

What organ? What stain? Be able to identify the types of cells

Answer 43

**Testis** in a **Convoluted Seminiferous Tubule** **Iron Hematoxylin** Gotta know different types of cells in spermiogenesis (Outer layer of squamous-looking cells around a seminiferous tubule = **Lamina Limitans**)

Question 44

What organ? What is 1? What's the tubule above #1? What stain?

Answer 44

**Mediastinum Testis** **1. Rete Testis of Haller** The tubule above #1 is probably a **straight seminiferous tubule** (tubuli seminiferi recti) **Iron Hematoxylin Stain**

Question 45

What tissue is this image from? What type of vessels make up the top half of the image? and the bottom half?

Answer 45

**Epididymis** Top half = **Ductus Epididymis** - note the pseudostratified columnar epithelium, with stereocilia Bottom half = **Ductus Efferentes Testis** with characteristic alternating cell height, simple cuboidal and simple/psuedostratified columnar epithelium, with microvilli, and kinocilia

Question 46

What type of vessel is this?

Answer 46

**Ductus efferentes testis**

Question 47

What vessel is this? What type of epithelium?

Answer 47

**Ductus Epididymis** **Pseudostratified Columnar Ciliated**

Question 48

What tissue is this from? What's the huge vessel in the middle? (you also should be aware of its layers) & What kind of Epithelium? What's the smaller (but still pretty big) vessel in the top-right of the image?

Answer 48

**Spermatic Cord** Big thing - **Ductus (or Vas) Deferens**, with **pseudostratified columnar ciliated epithelium** and **3 muscular layers** Top-right vessel - **Deferential Artery**, should always be near the ductus deferens

Question 49

Which tissue is this image from?

Answer 49

**Seminal Vesicle** Looks similar to the *ampulla of the oviduct*, especially without more in the frame

Question 50

What type of epithelium is here? What organ is this from?

Answer 50

**Simple Cuboidal** to **Columnar Epithelium** (it's variable in this organ, depending on testosterone level. Our green histo book p. 162 says simple cuboidal to simple columnar, but other sources say it's more between pseudostratified columnar and simple columnar, and I believe that is probably more correct than our book because just look at it) In the **Seminal Vesicle**

Question 51

Which organ is this image from? What type of gland/secretion is this?

Answer 51

**Prostate Gland** **Apocrine Gland** (although people have recategorized it to "**pseudo-apocrine**" because it's really more merocrine. Fucking scientists.) Has low cuboidal to columnar epithelium, widely dilated lumen, corpora amylacea/ prostatic stones, smooth muscle bundles within the connective tissue septa

Question 52

What tissue/region is this from? What type of epithelium in that lumen at the top? What are the blue arrows pointing at? What are the red stars showing?

Answer 52

**Penis - Corpus Spongiosum** **Stratified Columnar Epithelium** of Urethra Blue arrows = **Intimal Cushions of Ebner** inside the **Helicine Arteries** Red Stars = **Littre's Paraurethral Glands**

Question 53

What tissue is this from? What kind of glands do you see?

Answer 53

**Glans Penis** (foreskin) Probably the only place with one external and two internal layers stratified squamous keratinizing epithelium Has some (**holocrine) sebaceous glands** Notable features: **prepuce**, **navicular fossa, strat squam non-ker epithelium in urethra** (Having trouble finding any decent images for this; either it's shitty histology sections or it's mutilated dicks)