Detecting Proteins using Antibodies 1.1 Flashcards
1
Q
what are immunoassay techniques used for?
A
to detect and identify specific proteins
2
Q
what do immunoassay techniques use?
A
stocks of antibodies with the same specificity, known as monoclonal antibodies
3
Q
what is an antibody specific to the protein antigen is linked to?
A
a ‘chemical label’
4
Q
what is used after SDS-PAGE electrophoresis
A
western blotting
5
Q
describe western blotting
A
- separated proteins from the gel are transferred (blotted) onto a solid medium
- proteins can be identified using specific antibodies that have reporter enzymes attached
6
Q
what is ELISA?
A
an analytical technique which uses antibodies to detect the presence of an antigen in a solution
