Flashcards in DIAGNOSTIC STAINS Deck (76)
What are properties of Diagnostic Stains?
1) To enhance contrast
2) Special affinity for ocular tissues and cells
3) Acidic or basic
4) Chromatic differentiation depends on distinct absorption and emission spectrum
What are characteristics of Fluorescein?
Water soluable (Weak Dibasic xanthine acid)
Ionized in tears
orange-yellow-green in tear film
What is the absorption level in FLUORESCEIN?
AT 493 NM FOR cobalt blue
465 nm in Blood
What is the Emission rate in FLUORESCEIN?
AT 520 NM (YELLOW GREEN)
what is Seidel's sign?
Fluorescent green in bowman's membrane or aqueous humor
Due to Precipitation of the cornea
aquous humor leakage out of anterior chamber because there is a penetrating abrasion
Why is hepatic metabolism important with FLUO?
because, if you are giving orally, and the pt has liver disease, you are recognizing they are not going to metabolize to metabolize without any difficulty
what is QUENCHING?
this is when the FLUORESCEIN intensity goes down giving you false negative findings may result
Can Anesthetics can be quenching? If so, what is the order of the drugs from Least to Most?
(least) BAC and Benoxinate < Proparacaine < Tetracaine (most quenching)
What are the indications of the FLUORESCEIN?
Abrasion or ulcer and Edema
Alkaline burn: non soluble particles
Dry eye staining and tear breakup time (TBUT)
What confirms the Foreign body?
Siedel's sign confirms full thickness corneal penetration
What should be done always at first when evaluating abrasion or ulcer and edema?
Locate of anterior chamber for cells and flare always should be completed before staining
What does alkaline burn do?
Pt with Fluorescein dye will highlight the alkaline particles that are less soluble, you have remove all the particles, of the injury
What happens with the TBUT
with the FLUO dye, the normal healthy eye will make a nice uniform layer, if its normal it will be 10 seconds or more for TBUT, if its sooner, explain dryness
What is PUNCTATE KERATITIS?
inflammation on the corea, and its superficial that cuases small patches of keratitis
Penetrating the stroma causes?
FLUORESCEIN examination done in what type of lamp?
How to evaluate the RGP CL ?
Staining under lens is blue in absence of stain
Green edges means there is edge lift, which may begood
In ORTHO k - what is the objective in RGP?
to create 5 microns over corneal apex
How much thicker the layers need to be in a human eye to detect fluorescein?
20 microns or thicker
What kind of filter do you need to evaluate RGP?
Wratten 47, since RGPS may block UV from burton lamp
What is Epiphora? and what test do you use to evaluate this?
Epiphora = tears running down the cheek, due to obstruction of the naso lacrimal duct
What is Jones 1?
2% Fluorescein to ocular surface, blow through one side nostil (ipsilateral) after 5 mintues, if dye appears on the tissue the dye passed thorugh nasal lacrimal duct, if not then use JONES II
What is Jones II?
To confirm the location of obstruction of the canal
What is Aniline Tint Antidote?
methyl violet = which is a aniline dye that is used to permanent pencils spreads through ocular tissues and percipitated out by 2% sodium fluorescein
How much do you use to measure aqueous humar production?
0.5% oral fluorescein
- we are using the oral form, this will appear ant chamber and give info about ciliary body
-- impt GLAUCOMA
What is Filamentous Keratitis?
Inflammation of the cornea, that forms mucous type of epithelial filament that form fiber
there fore FLUO abosrs this very well you can use to identify the keratitis
How to recognize HSV KERATITIS?
it will have bulbar like endings or round endings
uses sodium FLUOrescien to recognize
when they dont have bulbar like endings,
Uses sodium fluorescein to recognize
what is applation tonometry?
it is a way to estimate the IOP PRESSURE