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Flashcards in DIAGNOSTIC STAINS Deck (76)
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1

What are properties of Diagnostic Stains?

1) To enhance contrast
2) Special affinity for ocular tissues and cells
3) Acidic or basic
4) Chromatic differentiation depends on distinct absorption and emission spectrum

2

What are characteristics of Fluorescein?

Water soluable (Weak Dibasic xanthine acid)
Ionized in tears
orange-yellow-green in tear film

3

What is the absorption level in FLUORESCEIN?

AT 493 NM FOR cobalt blue
465 nm in Blood

4

What is the Emission rate in FLUORESCEIN?

AT 520 NM (YELLOW GREEN)

5

what is Seidel's sign?

Fluorescent green in bowman's membrane or aqueous humor
Due to Precipitation of the cornea
aquous humor leakage out of anterior chamber because there is a penetrating abrasion

6

Why is hepatic metabolism important with FLUO?

because, if you are giving orally, and the pt has liver disease, you are recognizing they are not going to metabolize to metabolize without any difficulty

7

what is QUENCHING?

this is when the FLUORESCEIN intensity goes down giving you false negative findings may result

8

Can Anesthetics can be quenching? If so, what is the order of the drugs from Least to Most?

Yes
(least) BAC and Benoxinate < Proparacaine < Tetracaine (most quenching)

9

What are the indications of the FLUORESCEIN?

Foreign body
Abrasion or ulcer and Edema
Alkaline burn: non soluble particles
Dry eye staining and tear breakup time (TBUT)

10

What confirms the Foreign body?

Siedel's sign confirms full thickness corneal penetration

11

What should be done always at first when evaluating abrasion or ulcer and edema?

Locate of anterior chamber for cells and flare always should be completed before staining

12

What does alkaline burn do?

Pt with Fluorescein dye will highlight the alkaline particles that are less soluble, you have remove all the particles, of the injury

13

What happens with the TBUT

with the FLUO dye, the normal healthy eye will make a nice uniform layer, if its normal it will be 10 seconds or more for TBUT, if its sooner, explain dryness

14

What is PUNCTATE KERATITIS?

inflammation on the corea, and its superficial that cuases small patches of keratitis

15

Penetrating the stroma causes?

ULCER

16

FLUORESCEIN examination done in what type of lamp?

COBALT BLUE

17

How to evaluate the RGP CL ?

Staining under lens is blue in absence of stain
Green edges means there is edge lift, which may begood

18

In ORTHO k - what is the objective in RGP?

to create 5 microns over corneal apex

19

How much thicker the layers need to be in a human eye to detect fluorescein?

20 microns or thicker

20

What kind of filter do you need to evaluate RGP?

Wratten 47, since RGPS may block UV from burton lamp

21

What is Epiphora? and what test do you use to evaluate this?

Epiphora = tears running down the cheek, due to obstruction of the naso lacrimal duct

Jones test

22

What is Jones 1?

2% Fluorescein to ocular surface, blow through one side nostil (ipsilateral) after 5 mintues, if dye appears on the tissue the dye passed thorugh nasal lacrimal duct, if not then use JONES II

23

What is Jones II?

To confirm the location of obstruction of the canal

24

What is Aniline Tint Antidote?

methyl violet = which is a aniline dye that is used to permanent pencils spreads through ocular tissues and percipitated out by 2% sodium fluorescein

25

How much do you use to measure aqueous humar production?

0.5% oral fluorescein
- we are using the oral form, this will appear ant chamber and give info about ciliary body
-- impt GLAUCOMA

26

What is Filamentous Keratitis?

Inflammation of the cornea, that forms mucous type of epithelial filament that form fiber
there fore FLUO abosrs this very well you can use to identify the keratitis

27

How to recognize HSV KERATITIS?

it will have bulbar like endings or round endings
uses sodium FLUOrescien to recognize

28

SIMPLEX ZOSTER?

when they dont have bulbar like endings,
Uses sodium fluorescein to recognize

29

what is applation tonometry?

it is a way to estimate the IOP PRESSURE

30

why Fluorescein not good with tonometry?

becaue it underestimate it, therefore we need to use a ANESTHETIC , since the probe does touch the surface of the cornea