DIFFERENT SPECIMEN COLLECTION

Question 1

blood to culture media ration

Answer 1

1:10

Question 2

colony count of _______________ and above is indicative of UTI

Answer 2

100,000 CFU/mL

Question 3

types of UTI

Answer 3

• cystitis
• urethritis
• pyelonephritis
• pyelitis
• glomerulonephritis

Question 4

these findings suggests that the sputum is unsatisfactory:

Answer 4

• more than 10 squamous epithelial cells/LPF
• less than 25 polymorphonuclear leukocytes

Question 5

recommended volume for CSF

Answer 5

> /= 1ml

Question 6

when do you collect specimen for urine culture

Answer 6

early morning

Question 7

cotton swabs with wooden shaft are not reccomended for:

Answer 7

fastidious bacteria (Neisseria)

Question 8

Aerobic bacteria are collectef from:

Answer 8

• upper respiratory tract
• nasopharyngeal
• external ear
• eye
• genital tract

Question 9

what should be done if patient is having trouble expectorating sputum

Answer 9

induce by inhaling saline mist

Question 10

for lesions, wound and abscess, collect the specimen from the

Answer 10

advancing margin

Question 11

bood cultures should be incubated in what temperature

Answer 11

37 c

Question 12

proper volume of sputum to be collected

Answer 12

5-10 mL

Question 14

for deep wound collection

Answer 14

aspirate

Question 15

recommended volume for blood specimen collection for adults and neonates

Answer 15

• 20 ml
• 1 - 20 ml

Question 16

best time to collect blood specimen

Answer 16

before and after fever spike

Question 17

as per National Tuberculosis Control Program, for diagnosis of pulmonary tuberculosis, how many specimen should you collect

Answer 17

2 (spot and early morning) in seperate days

Question 18

swabs use for gonococcal urethritis, vaginosis, vaginatis

Answer 18

Urogenital swabs

Question 19

Main anticoagulant for blood culture

Answer 19

SPS (0.025-0.030%)

Question 20

manner of collection for tissue specimens

Answer 20

biopsy

Question 21

when will youu collect specimen?

Answer 21

acute phase of the infecction

Question 22

other specimens for PTB

Answer 22

• transcheal aspirate
• BAL
• Bronchial washings

Question 23

CSF is collected in how many tubes and what ube is for bacteriology

Answer 23

3 (2nd for Bacteriology)

Question 24

collect specimens prior to administration of ___________

Answer 24

antibiotics

Question 25

culture used for isolation of group A Streptococcus

Answer 25

throat culture

Question 26

body fluids are collected via

Answer 26

needle aspiration

Question 27

E.coli and other enterics (Proteus, Enterobacter, Klebsiella), Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, S. saprophyticus are found in what specimen

Answer 27

Urine

Question 28

swabs for viral culture:

Answer 28

- cotton - dacron

Question 29

collect stool specimen for how many days?

Answer 29

3 consecutive days

Question 30

for lesions, wound and abscess what will you identify first?

Answer 30

exact anatomic site

Question 31

for diagnosis of meningitis or menigoencephalitis

Answer 31

CSF

Question 32

how many blood culture should be done and how many hours apart

Answer 32

2-3 culture an hour apart

Question 33

other coagulants used for bood culture:

Answer 33

- heparin - sodium amyosulfate - sodium citrate

Question 34

Bartlett's scoring is not applicable for these organisms:

Answer 34

- Mycoplasma - Chlamydophila - Mycobacteria - fungi - Legionella - Viruses

Question 35

where do you collect Blood specimen?

Answer 35

2 - 3 different sites (left and right) within 24 hours

Question 36

H. influenzae, N. meningitidis, S.pneumoniae, S. agalactiae, Listeria can be found in what specimen

Answer 36

CSF

Question 37

C. jejuni, Salmonella, Dhigella, E.coli, Vibrio, Y. enterocolitica, L. monocytogenes, C. difficile, MAC are found in what specimen:

Answer 37

Stool

Question 38

what will you use for anaerobic bacteria

Answer 38

aspirates

Question 39

Gasric tubes used for gastric specimens

Answer 39

- Levine - Rehfuss

Question 40

Bartlett's total score of zero means?

Answer 40

lack of inflammatory reaction or contamination with saliva

Question 41

use sterile containers except in:

Answer 41

stool collection

Question 42

specimen of choice for the diagnosis of GI pathogens

Answer 42

stool

Question 43

swabs for bacterial culture

Answer 43

- dacron - calcium alginate - rayon

Question 44

manner of collection for CSF

Answer 44

Lumbar tap (3rd-4th lumbar vertebra)

Question 45

for diagnosis of lower respiratory tract infections (bacterial pneumonia, PTB)

Answer 45

sputum

Question 46

body fluids include:

Answer 46

- abdominal fluid - amniotic fluid - ascitic fluid - bile - synovial fluid - pleural fluid - pericardial fluid

Question 47

can be employed to check the quality or adequacy of the sputum

Answer 47

Bartlett's grading system

Question 48

type of urine collection during cystoscopy

Answer 48

- straight catheterized urine - suprapubic aspirates

Question 49

stain for sputum

Answer 49

gram

Question 50

types of biopsy

Answer 50

- excisional - incisional

Question 51

Media of choice for Blood Culture

Answer 51

broth - BHI - TSB - BIphasic Media - Thioglycollate broth - commerciall available bottles Isolator system

Question 52

how many swab sample should you get

Answer 52

2

Question 53

for diagnosis of fever of unknown origin, septicemia, bacteremia, typhoid fever, SBE

Answer 53

Blood

Question 54

what will you use for aerobic bacteria

Answer 54

swab

Question 55

culture used for diagnosis of pertussis, middle ear infections, carrier state of S. aureus, N.meningtidis, H. influenzae

Answer 55

Nasopharyngeal cultures

Question 56

what should we avoid as urine specimen:

Answer 56

- random voided urine - foley catheters