PHYSICAL METHODS

Question 1

for critical sterilization _______ filter must be used

Answer 1

0.22 um

Question 2

Ionizing radiations:

Answer 2

Gamma rays or electron beams

Question 3

filtration of liquids through sintered glass

Answer 3

morton

Question 4

machine used for tyndallization

Answer 4

arnold’s sterilizer

Question 5

outlawed the incinirator

Answer 5

RA 8749 Clean air act of 1999

Question 6

mechanism of action od radiations

Answer 6

alkylation of nucleic acids

Question 7

for disposal of hospital waster

Answer 7

incinirator

Question 8

insipissation temp and duration

Answer 8

75-80 c for 2hrs on 3 consecutive days

Question 9

moist heat principle

Answer 9

coagulation of proteins

Question 10

dehydration

Answer 10

dessication

Question 11

size HEPA filter can remove

Answer 11

> 0.3 um

Question 12

removal of water to stop metabolic action of bacteria

Answer 12

dessication

Question 13

filtration of liquids through unglazed porcelain

Answer 13

chamberland-pasteur

Question 14

is exposure to low temperature bactericidal or bacteriostatic

Answer 14

bacteriostatic

Question 15

most commonly used metthod

Answer 15

HEAT

Question 16

free flowing steam

Answer 16

tyndallization

Question 17

for sterilization of inoculating loops, needles and mouth of tubes

Answer 17

bunsen burner

Question 18

through plamolysis

Answer 18

high osmotic pressure

Question 19

filtration of liquids through asbestos pad

Answer 19

seitz

Question 20

boiling temp and duration

Answer 20

100 c for 10-15 mins

Question 21

Non ionizing radition:

Answer 21

UV rays

Question 22

form of moist heat disenfection that doesent kill pores

Answer 22

Boiling

Question 23

using high concentrations of salt/sugar

Answer 23

high osmotic pressure

Question 24

principle of dry heat

Answer 24

oxidation

Question 25

Autoclave temp, psi, time

Answer 25

121 c, 15 PSI, 15 mins

Question 26

methods of pasteurization and their temp and duration

Answer 26

- Batch method (63 c for 30 mins) - Flash method (72C for 15 seconds) - High tempt Short time (72-140c then 72c for <5 secs)

Question 27

for sterilizing glasswares

Answer 27

HOT AIR oven

Question 28

for sterilization of high protein media

Answer 28

insipissation

Question 29

eliminates food borne pathogens responsiblle for food spoilage

Answer 29

pasteurization

Question 30

filtration of liquids through cellulose ester

Answer 30

membrane filters

Question 31

HEPA meaning

Answer 31

High Efficiency Particulate Air Filter

Question 32

seperation of bacteria from liquids or air

Answer 32

filtration

Question 33

short wavelenght but high energy

Answer 33

gamma rays

Question 34

long wavelength but low energy

Answer 34

uv rays

Question 35

steriization of disposable materials in the medical industry

Answer 35

gamma rays

Question 36

use for disinfection of surfaces

Answer 36

UV rays

Question 37

filtration of liquids through diatomaceous earth

Answer 37

berkefeld

Question 38

tyndallization temp and duration

Answer 38

100c for 30 mins for 3 days

Question 39

temperature requirements for glassware sterilization

Answer 39

160-180 c for 1.5-3 hrs