Digestion in ruminants Flashcards

1
Q

How does ruminants accomplish cellulose digestion?

A

Indirectly through the microorganisms growing in the GIT, particularly in the forestomach.

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2
Q

What does the microorganisms provide?

A

The essential cellulase enzymes to decompose plant material.

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3
Q

What does microorganisms produce?

A

Many nutrients required bu polygastric animals.

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4
Q

What is fermentation?

A

The process within a ruminant’s stomach in which FFA, protein, vitamins and other nutrients are synthesized to mee the metabolic demand.

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5
Q

What is normal physiological colour of rumen fluid?

A

Green

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6
Q

Bright/yellowish colour of rumen fluid is a sign for?

A

Acidosis

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7
Q

Dark colour of rumen fluid is a sign for?

A

Alkalosis

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8
Q

Trace of…….. in ruminal fluid is pathogenic?

A

Blood

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9
Q

What is pH of ruminal fluid?

A

6,2-6,8

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10
Q

Which temperature is needed for microorganisms to function?

A

39-41 C

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11
Q

What environment is needed for microorganisms to survive?

A

strictly anaerobic

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12
Q

How many species of MO’s are found in ruminal fluid?

A

200 species

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13
Q

Two main types of MO’s in rumen?

A

Cilliata and flagellata

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14
Q

Types of cilliated MO’s?

A

Holotricha –> all over body

Entodiniomorpha –> cillia in branches (diplodinium, entodinium, epidinium)

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15
Q

Other name for protozoa?

A

Infusaria

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16
Q

How many MO’s in rumen?

A

200-400 x 10^6 /mL

17
Q

Which types of MO’s in rumen?

A
Bacteria
Protozoa
Yeast
Mold
Fungi
18
Q

How is reduction test of ruminal fluid performed?

A

Add methylen blue to ruminal fluid and put in tempered bath. Measure time for decomposition of MB.
Living/moving protozoa will reduce the colour of methylen blue. Dead will not decomposite it = blue colour.

19
Q

How many species of bacteria in rumen?

A

200 species

20
Q

Function of bacteria?

A

Utilization of starch

21
Q

Number of bacteria in rumen?

A

10^9 - 10^12 / 1 mL of ruminal fluid

22
Q

How is amount of bacterias regulated?

A

Protozoas eat bacteria and regulate amount

23
Q

Function of yeast?

A

Use oxygen, help maintaining anaerobic environment.

24
Q

How much Volatile FA are produced in rumen / day?

A

2-4,5 kg. Saccharides –> pyruvate –> Volatile FA–> lactic acid

25
Precursor of acetic acid?
Milk fat
26
Precursor of propyonic acid?
glucose
27
Precursor of butyric acid?
milk fat
28
What is spesial in ruminant digestion?
Fermentation process in rumen can create all essential AA. Do not have to ingest them.
29
Main product of proteolysis?
NH4+ --> urea --> 1. kidnes and urine 2. saliva and back to rumen.
30
pH of saliva?
pH 8 --> alkalic because of bicarbonate
31
Why is ruminant saliva alcalic?
To function as a buffer system for the acidity in rumen.
32
Formula for Total Count (TC) of living protozoa in ruminal fluid?
TC= counted protozoa x 3 (dillution)x 320 (index for FR chamber) = 200-400 x 10^6 / mL
33
What is used to count living protozoa in ruminal fluid?
Fuchs-Rosenthal chamber (count 16 large squares)
34
How is the dillution used for the count?
1 mL RF + 2 mL Methylen blue (Dillution = 3)