Diversity of Cells and Their Function

Question 1

How many steps are involved to be able to see different tissue components and what are they

Answer 1

3
Tissue Processing
Embedding and Sectioning
Staining

Question 2

What are the steps used to be able to see different tissue components

Answer 2

Tissue processing
Embedding and Sectioning
Staining

Question 3

Why is tissue often fixed is the tissue processing step.

Answer 3

Tissue is often fixed to preserve it in as life-like state as possible. Usually a chemical that cross links proteins such as formalin is used

Question 4

What is staining

Answer 4

Staining is a process by which a tissue section is coloured.

Question 5

How many main types of stains are there

Answer 5

3. acidic stains
   basic stains
   neutral stains

Question 6

What colour are acidic stains

Answer 6

The acidic stains are negatively charged and combine wit positively charged particles. They are red or pink in colour. example=eosin. tissue that stain red with acidic dye are termed acidophilic.

Question 7

What colour are basic stains

Answer 7

Basic stains are positively charged, and combine with negatively charged particles. They are blue or dark purple in colour. example=Hematoxylin & toluidine blue are the commonly used ones.