DNA Extraction Flashcards
1
Q
Break open the cells by disrupting the cell membrane
A
Lysis
2
Q
The liquid solution spins at high speed which forms two layers
A
Centrifugation
3
Q
This is added to the pellet to remove the contaminants
A
Absolute Ethanol (96% EtOH)
Washing Step
4
Q
The pellet is [blank] at a moderately warm temperature
A
Air-dried
Drying Step
5
Q
It binds DNA to the silica gel membrae
A
Buffer Solution
6
Q
It causes the DNA to precipitate
A
Ethanol
7
Q
It removes other impurities in the silica gel membrane
A
Washing
8
Q
It removes the nucleic acids from the silica gel membrane, allowing the DNA to flow through
A
Elution
9
Q
Spin-column Method Steps
A
- Lyse - break open the cells
- Binding - binding of DNA to the silica gel membrane
- Wash - washin the DNA bound to the silica gel membrane
- Elute - elution of DNA
