Polymerase Chain Reaction Flashcards
1
Q
Reagents for PCR
A
- DNA Template
- Buffer solution
- Primers
- DNA Polymerase
- DNA Nucleotides
and others
2
Q
Used to select the region to be copied, usually 18-25 nucleotides long
A
DNA Primers
3
Q
Synthesizes the new strand of DNA
A
Thermostable DNA Polymerase
Taq Polymerase
4
Q
Ensures ideal PCR condition, often contains Tris-HCL as well as enhancers
A
PCR Buffer
5
Q
deoxynucleotide triphosphates, nucleotides added during the extension phase of PCR
A
Nucleotides (dNTPs)
6
Q
Procedure
Hydrogen bonds are broken separating the strands
A
Denaturation
7
Q
Procedure
DNA primers attach to the specific sites on the DNA Template
A
Annealing
8
Q
Procedure
DNA polymerase synthesizes the new strands
A
Extension
9
Q
Uses for PCR
A
- DNA Fingerprinting
- Disease Diagnosis
