DNA replication Flashcards
1
Q
helicase role
A
mechanically breaks the hydrogen bonds (unzips the molecule)
2
Q
new nucleotides —— according to complimentary base pairing fules
A
anneal
3
Q
dna polymerase role
A
catalyses condensation of the phosphodiester bonds between adjacent nucleotides on the new strand
4
Q
direction of dna polymerase
A
5’ to 3’
5
Q
the leading strand and lagging strand are —–
A
antiparallel
6
Q
replication fork
A
where the hydrogen bonds are currently being broken
7
Q
experiment that discovered DNA replication is a semi-conservative process
A
-used autotroph bacteria
- only supplied the bacteria with N15 for a year
- extracted DNA and centrufuged it
- transferred bacteria to N14 for generation time (20 minutes)
- centrifuged DNA again
- then left for another 20 minutes
- centrifuged DNA
