methods of studying cells Flashcards
1
Q
for cell fractionation, solution must be…
A
cold to reduce enzyme activity
isotonic to prevent osmosis
buffered to prevent damage to organelles
2
Q
steps of cell fractionation
A
homogenisation, filtration, ultra centrifugation/ differential centrifugation
3
Q
calibrating eyepeice graticule
A
use a stage micrometer to assign a quantitative length to the arbitrary graticle units
4
Q
——- nm =1mm
A
1 million
5
Q
—–um =1mm
A
1000
