DNA Replication

Question 1

Bacteria have

Answer 1

circular and relatively smaller genomes

Question 2

Bacteria contain ___ origin of replication (oriC)

Answer 2

one

Question 3

replication forks

Answer 3

DNA is unwound and these are the points in which replication is happening

Question 4

Replication for originally appears at theorigin of replication and the spreads out ____ resulting in ___ replication forks

Answer 4

Bidirectionally
2

Question 5

Polymerase

Answer 5

formation of a polymer, repeating molecules

Question 6

DNA polymerases use energy from dNTPS to build

Answer 6

complementary sequence of DNA

Question 7

Two terminal phosphates are cleaved releasing pyrophosphate and incorporating a

Answer 7

dNMP into the DNA molecule

Question 8

Chain elongation in the

Answer 8

5’-3’ direction

Question 9

DNA polymerase III

Answer 9

DNA synthesis during replication and proofreading

Question 10

DNA polymerase I

Answer 10

Filling gaps and removing primer, DNA repair

Question 11

Active forms of DNA polymerase III is called

Answer 11

holoenzyme

Question 12

The length of DNA that is replicated is referred to as the

Answer 12

replicon

Question 13

holoenzyme- core enzyme with catalytic function

Answer 13

5’-3’ polymerization
3’-5’ exonuclease

Question 14

Sliding clamp loader and DNA clamp

Answer 14

processivity and ATP hydrolysis

Question 15

___ is needed to start synthesis for DNA polymerase

Answer 15

primer

Question 16

RNA primers must be removed and replaced with

Answer 16

DNA

Question 17

Gaps in backbone must be

Answer 17

sealed

Question 18

DNA helicase continuously separates

Answer 18

DNA strands

Question 19

Sing-Stranded binding proteins (SSBs)

Answer 19

bind and coat ssDNA molecules to protect ssDNA and inhibit dsDNA from reforming

Question 20

supercoiling

Answer 20

coiling tension

Question 21

DNA topioisomerase (gyrase)

Answer 21

cleave the DNA backbone using ATP

Question 22

An RNA polymerase (primase) adds a short ___ to which DNA pol III can add DNA for replication

Answer 22

RNA primer

Question 23

okazaki fragments are present on the

Answer 23

discontinuous lagging strand

Question 24

DNA pol I removes the

Answer 24

RNA primer and synthesizes new DNA

Question 25

DNA ligase joins together

Answer 25

okazaki fragments repairing the gap in the backbone

Question 26

Telomeres

Answer 26

protect the ends from nucleases

Question 27

Telomerase

Answer 27

extend the length of dsDNA

Question 28

Is telomerase active in most cells?

Answer 28

no