DNA5 Flashcards
(19 cards)
In translation, where does recognition of tRNA happen?
A-site; decoding site
In translation, where does catalysis happen?
P-site; transferase center
As a whole unit, what ultracentrifuge weight does the translational rRNA have?
70s
What’s the ultracentrifugal weight of small unit of translational rRNA?
30S
What’s the ultracentrifugal weight of the large subunit of translational rRNA?
50S
What is the role of the 30S subunit during translation?
hold mRNA
what is the role of the 50S subunit in translation?
catalyze the peptide bond formation
Translocation and binding of tRNA during translation is dependent on what?
GTP energy source
The ….. ….. signals the end of protein synthesis.
release factor
what charges tRNA with the correct amino acid?
tRNA Synthetases
What marks an activated compound that is ready for transfer during translation?
formylation
What is the start sequence of translation?
formylated methionine
What reaction is catalyzed at the active site of the ribosome for translation?
lactam condensation
How many proteins are in the prokaryotic ribosome?
55
How many proteins are in eukaryotic ribosomes?
79-80
There are no …… in the peptidyl transferase of the ribosome
proteins
What happens to the ribosome in cells when it stalls due to stress factors?
rescue factors (in bacteria) dissociate translationally inactive ribosomes into their subunits
What are two recycling factors found in bacteria?
GTPase and HflXr
What is ribosomal hibernation?
An inactive state in which ribosomes are dissociate and bind to ‘hibernation’ factors
