Drosophila Eye Development Flashcards

(38 cards)

1
Q

What are imaginal discs?

A

Areas which develop into the mouth, eye etc

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2
Q

When are imaginal discs set side?

A

In early embryogenesis

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3
Q

What is the haltere?

A

A balance organ

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4
Q

How many cells gather to form an imaginal disc?

A

Around 30

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5
Q

How big do structures from imaginal discs grow?

A

To about 50,000 cells

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6
Q

What is the phenotype of a Pax 6 mutant?

A

They have no eyes

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7
Q

What is eyeless?

A

A homeobox transcription factor homologous to vertebrate PAX6

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8
Q

Eyeless PAX6 is both … and … to make an eye

A

necessary

sufficient

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9
Q

PAX6 misexpressed in a fly induces what?

A

An ectopic eye

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10
Q

How many flies do drosophila have?

A

5 - 2 large and 3 small

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11
Q

What are the flies small eyes called?

A

Ocelli

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12
Q

What are ocelli used for?

A

Sense the flies orientation ie where they are in the sky

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13
Q

Each large eye consists of how many ommatidia?

A

800

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14
Q

What are ommatidia?

A

each of the optical units that make up the compound eye of an insect.

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15
Q

Why are the ommatidia hexagonal?

A

Makes them efficient in their packaging

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16
Q

Each ommatidia is associated with what?

A

A bristle involved in the cleaning process

17
Q

Why are the fly eyes curved?

A

To give them a wide plane of view

18
Q

Each ommatidia consists of:

A
8 photoreceptors
4 cone cells
2 primary pigment cells
6 secondary pigment cells
3 tertiary pigment cells
19
Q

Why do fly eyes contain red granules around the lens?

A

They stop light from other places being scattered so light is focused in a straight line on the ommatidia

20
Q

Why does a white mutant have worse vision than a wildtype fly?

A

The white mutant has no red granules so the light scatters and confuses things

21
Q

What are rhabdomeres?

A

Light sensitive structures
They trigger neuronal input
Made of microvilli packed with rhodopsin

22
Q

How many rhabdomeres are there?

23
Q

What is the role of the equator across the eye?

A

It senses the horizon

24
Q

Where do R1 to R6 axons project into?

25
Where do R7 and R8 photoreceptors project to?
The medulla
26
Why can a single object be seen by many rhabdomeres?
It increases resolution
27
What do flies respond to?
Movement
28
When does most growth of the eye field occur?
The third instar
29
Photoreceptors are preceded by what?
The morphogenetic furrow
30
Describe the morphogenetic furrow
Rich in actin Moves across the disc from the posterior to the anteror Has very few photoreceptors
31
Which is the first cell to be recruited in the ommatidia?
R8 - they are born with in the morphogenetic furrow
32
How are R8 cells recruited?
Using a process of lateral inhibition through Notch singalling - this inhibits other cells to become R8 cells
33
Describe the order which Rhabdomeres are recruited
1) R8, R2 and R5 secrete EGF pathway ligand Spitz 2) R3, R4 , then R1 and R6 recruited 3) R7 is the last 4) Then cone cells are recruited after
34
Mutants lacking R7 can not see what?
UV light | This means they go towards white light rather than UV light
35
Where are R8 cells born?
The morphogenic burrow
36
Mutations in which genes causes the loss of R7?
Boss and sevenless
37
What does the gene sevenless do?
Trans membrane receptor kinase Activator of the Ras/Raf/Erk pathway Expressed in R8,1,6,4,3,7 and con cells
38
What does Boss do?
A membrane tethered ligand specific to sev | Expressed eclusively in R8