Effect of Ph on Amylase

Question 1

What does Amylase do?

Answer 1

Breaks down **starch **into simple sugars.

Question 2

2 problems

What are the problems with the practical and how can they be negated?

Answer 2

1. Sample taken every 30s so we only have an approximate time for reaction completion.
   Fix: Take sample more frequently e.g. every 10s
2. Colour change may be gradual.
   Fix: Ask several people to see when colour change occurs.

Question 3

8 Steps. Gonna need a lot.

What are the steps for testing the effect of ph on Amylase?

Use terms including: Iodine, Spotting tile, Well, Starch, Amylase, Starch, Buffer, Test Tubes, Waterbath, Stirring Rod, Repeat

Answer 3

1. Place one drop of iodine solution into each well of spotting tile.
2. Add 2cm^3 of starch solution, 2cm^3 of Amylase solution and 2cm^3 of ph 5 buffer solution to a test tube each. (Buffer solution controls ph)
3. Place test testubes in 30 degrees celsius waterbath and leave for 10 minutes to reach correct temperature.
4. Combine 3 solutions into one test tube and mix with stirring rod.
5. Return to waterbath and start stopwatch.
6. Take sample after 30s using stirring rod and transfer one drop of solution to a well on spotting tile containing iodine. (Iodine will turn blue black)
7. Repeat until Iodine remains orange ( Starch no longer present as Amylase has broken down all starch into simple sugars).
8. Repeat experiment using different ph buffers e.g. ph 6, 7,8