Efficacy Flashcards
Microbiology can’t be used as a diagnostic why?
B/c the microbiota varies between each individual
Bacterial Culturing
Gold Standard
-Limitations: can only grow live bacteria, takes long time for growth, sensitivity is low
Immunodiagnostic Methods Used mainly to detect
Aa and Pg
Immunodiagnostic Methods limitations
Costly and complex
More commonly used in lab not clinic
Molecular biology Techniques
PCR-high sensitivity and specificity
Immunology
Biochemical analysis as part of periodontal diagnosis
Sources: GCF, Saliva
Immunology: GCF
Most commonly used
-evaluate host derived enzymes and their inhibitors, byproducts of tissue breakdown, inflammatory mediators and host response modifiers
Immunology limitations
Selection of teeth and sites is often diffiucult
-immunoassay only test a single cytokines at a time
Immunology: Saliva
Abundant fluid and easy to collect and store
-highly enriched content of disease biomarkers
Genetics dont predict
Fail to determine when periodontal destruction will occur
-identify general risk factors
Tissue Breakdown Products
Destruction of collagen
-detects types of collagen, proteoglycan, non collagen proteins
Tissue Breakdown Products: elevated levels of
Hydrooxyproline
Glycoaminoglycans
Osteocalcin
Type 1 Collagen can be found in the GCF forms sites with periodontitis
Cone Beam Computed Tomography
Very Specific but lack sensitivity
-CBCT is promising for perio applications such as infrabony defects perio cysts furcation defects thickness of palatal masticatory mucosa
Infrared Spectroscopy Can measure the total
Can measure the total contents of GCF
Infrared Spectroscopy requires
Only small sample volumes and minimal training for operator
Reagent free
