Elek Molnar

Question 1

Exclusive pre-synaptic mGluRs

Answer 1

mGlu4 + mGlu7 = Gi/o (group 3)

Autoreceptors - regulate neurotransmitter release

Question 2

NMDAR subtype found abundantly at extra-synaptic locations

Answer 2

2B-containing NMDARs

Question 3

70% NMDARs

Answer 3

2x GluN1 (glycine) 
2x GluN2 (glutamate)

Question 4

Glutamatergic receptora cloned via expression cloning

Answer 4

GluA1
GluN1
mGlu1

Question 5

AMPAR sub-type expressed exclusively in the cerebellum

Answer 5

GluA4

Question 6

Developmental changes: NMDAR subunit expression

Answer 6

GluN1 + GluN2B –> GluN1 + GluN2A
2A = faster + more dynamic NMDARs but less calcium permeable

2B expressed in the embryo; 2A expressed after birth

Question 7

Which NMDAR subunit dictates NMDAR biophysical properties

Answer 7

GluN2

ie. Agonist affinities, open probability, deactivation kinetics

Question 8

Developmental changes: kainate receptor subunit expression

Answer 8

GluK1-3 –> GluK1-3 and GluK4-5

Question 9

An example of modifying an existing, mature AMPAR

Answer 9

GluA1 - P @ Ser831 by CamKII/PKC

Increase single channel conductance

Question 10

LTD

Answer 10

GluA2: P @ Ser880 by PKC - enhance GluA2-PICK1

Tau: P @ Ser396 - required for LTD

GluA1: deP @ Ser845 by S/TP - internalinse GluA1
(NMDAR influx activates S/TP)

mGluR-dependent LTD
mGlu5 activates PTP = deP tyrosine residues on GluA2 - favour internalisation

Question 11

LTP

Answer 11

GluA1: P @ Ser818 by PKC - enhance GluA1-4.1N interaction (inserting AMPARs into the synapse)