ELISA Flashcards
(22 cards)
What does ELISA stand for?
Enzyme linked immuno-sorbant assay
What is the purpose of an ELISA test?
To detect the presence of antibodies or antigens in a sample
True or False: ELISA is a commonly used diagnostic test in medicine.
True
Fill in the blank: ELISA is a ____________-based assay technique.
enzyme
What type of enzyme is commonly used in ELISA tests for detection purposes?
horseradish peroxidase
What is the color change mechanism used in ELISA tests?
substrate conversion by the enzyme
What are the two main types of ELISA tests?
indirect ELISA and sandwich ELISA
What is the key difference between indirect ELISA and sandwich ELISA?
The way the antigen is detected
True or False: ELISA tests are quantitative in nature.
True
What is the typical incubation time for an ELISA test?
1-2 hours
What is the purpose of adding a stop solution in an ELISA test?
To halt the enzyme reaction
Fill in the blank: The signal in ELISA tests is typically __________ to the amount of antigen present.
proportional
What is the advantage of using ELISA tests in terms of sensitivity?
High sensitivity
Which type of ELISA test is more commonly used for detecting antibodies?
Indirect ELISA
What is the role of the substrate in an ELISA test?
To produce a color change for detection
True or False: ELISA tests can be automated for high-throughput screening.
True
What is the main advantage of using ELISA tests over other immunoassay techniques?
High specificity
What does the optical density in an ELISA test indicate?
The amount of antigen present
Fill in the blank: ELISA is a widely used technique in __________ research.
biomedical
What is the main limitation of ELISA tests?
Cross-reactivity with similar antigens
What are some common applications of ELISA tests?
Diagnosis of infectious diseases, detection of allergens, monitoring autoimmune diseases
True or False: ELISA tests have revolutionized the field of diagnostics.
True
