EMBEDDING Flashcards
the process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium, which is then allowed to solidify
EMBEDDING
Embedding is also known as…
CASTING, BLOCKING
Paraffin embedded tissues are arranged at the bottom of the mold together with their proper labels and immersed in melted paraffin at what temperature?
5-10°C ABOVE its MELTING POINT
the wax is cooled rapidly in a refrigerator at ____ or immersed in __________ to solidify
-5°C; COLD WATER
the process by which a tissue is ARRANGED IN A PRECISE POSITION IN THE MOLD DURING EMBEDDING, on the microtome before cutting, and on the slide before staining
ORIENTATION
TYPES OF BLOCKING-OUT MOLDS
consists of 2 L-SHAPED PIECES of heavy brass or metal
LEUCKHART’S EMBEDDING MOLDS
TYPES OF BLOCKING-OUT MOLDS
made up of INTERLOCKING PLATES resting on a flat metal base, forming SEVERAL COMPARTMENTS
COMPOUND EMBEDDING UNITS
TYPES OF BLOCKING-OUT MOLDS
consist of special stainless steel base mold fitted with a PLASTIC EMBEDDING RING which later serves as the block holder during cutting
PLASTIC EMBEDDING RINGS and BASE MOLD
TYPES OF BLOCKING-OUT MOLDS
available in 3 different sizes, PEELED OFF ONE AT A TIME
PEEL-AWAYS
TYPES OF BLOCKING-OUT MOLDS
used in ordinary refrigerators RECOMMENDED FOR BUSY ROUTINE LABORATORIES
PLASTIC ICE TRAYS
TYPES OF BLOCKING-OUT MOLDS
cheap and easy to make; used for EMBEDDING CELLOIDIN and PARAFFIN BLOCKS
PAPER BOATS
the process in which tissues are FIRST INFILTRATED WITH CELLOIDIN and SUBSEQUENTLY EMBEDDED IN PARAFFIN MASS
DOUBLE EMBEDDING METHOD
in Double Embedding Method, the tissues are first infiltrated with ______ and subsequently embedded in __________ mass
CELLOIDIN; PARAFFIN
C comes before P, wow!
this method has SUPERIOR RESULTS FOR LIGHT MICROSCOPIC STUDIES, particularly in hard tissues and for HIGH RESOLUTION LIGHT MICROSCOPY OF TISSUE SECTIONS
PLASTIC / RESIN EMBEDDING
PLASTICS are classified into what 3 types?
- EPOXY PLASTICS
- POLYESTERS PLASTICS
- ACRYLIC PLASTICS
“EPA”
PLASTICS:
balance mixture of EPOXY PLASTIC, CATALYSTS, and ACCELERATORS
EPOXY PLASTICS
PLASTICS:
slow infiltration because of LARGE MOLECULE EPOXY PLASTIC
BISPHENOL A
PLASTICS:
have lower viscosity; often sold as MIXTURES OF ISOMERS
GLYCEROL
PLASTICS:
can be obtained PURE, have very low viscosity, INFILTRATE FASTEST
CYCLOHEXENE DIOXIDE
PLASTICS:
other term for BISPHENOL A
ARALDITE
A for ARALDITE 🌟
PLASTICS:
other term for GLYCEROL
EPON
PLASTICS:
other term for CYCLOHEXENE DIOXIDE
SPURR
PLASTICS:
ORIGINALLY INTRODUCED FOR ELECTRON MICROSCOPY in the mid-1950; superseded by more superior oxides and are now seldomly used
POLYESTER PLASTICS
PLASTICS:
made up of ESTERS OF ACRYLIC or METHACRYLIC ACID and are used EXTENSIVELY FOR LIGHT MICROSCOPY
ACRYLIC PLASTICS